Mycobacterium phage Hyperbowlee
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Detailed Information for Phage Hyperbowlee
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySieun Ruth Lee
Year Found2014
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.7884 N, 86.1001 W Map
Discovery NotesThe phage was discovered on August 25, 2014 at 6:57pm. The ambient temperature was 82°F and the soil depth was 2cm. The soil was moist and located right underneath some bushes in front of Martha Miller Center on Hope College Campus.
Naming NotesMy friends have bestowed upon me the nickname hyperbowlee because I'm hyper most of the time and I had a bowl cut in 7th grade. It is also a pun on the word hyperbole.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 8, 2019
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1040
Genome length (bp)59905
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

Topper (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionHope College
Annotation StatusIn GenBank
Plaque NotesThe pure phage stock of Hyperbowlee resulted in a clear, circular plaque with fuzzy edges (Figure 1). Its morphology is very similar to that of D29; however, their size differences assure us that it is distinct from D29, which is smaller than Hyperbowlee. The plaque is also a little more turbid than that of D29. Hyperbowlee’s plaque ranged from 2 to 2.5 mm in diameter at 24 hours and 4.2 to 5.2 mm in diameter at 48 hours. Its maximum detected size was 5.6 mm in diameter. Its growth rate was thus determined to be approximately 0.102 mm/hour. Hyperbowlee was isolated and purified at 32°C and was able to grow at 42°C.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Hyperbowlee_1

Hyperbowlee_2

Hyperbowlee_3

Hyperbowlee_4

Hyperbowlee_6

Hyperbowlee_7

Hyperbowlee_8
(terminase)

Hyperbowlee_9
(portal protein)

Hyperbowlee_10
(MuF-like minor capsid protein)

Hyperbowlee_11

Hyperbowlee_12
(scaffolding protein)

Hyperbowlee_13
(major capsid protein)

Hyperbowlee_14
(head-to-tail adaptor)

Hyperbowlee_15
(head-to-tail stopper)

Hyperbowlee_16

Hyperbowlee_17
(tail terminator)

Hyperbowlee_18
(major tail protein)

Hyperbowlee_19
(tail assembly chaperone)

Hyperbowlee_20
(tail assembly chaperone)

Hyperbowlee_21
(tape measure protein)

Hyperbowlee_22
(minor tail protein)

Hyperbowlee_23
(minor tail protein)

Hyperbowlee_24
(minor tail protein)

Hyperbowlee_25
(minor tail protein)

Hyperbowlee_26
(minor tail protein)

Hyperbowlee_27
(minor tail protein)

Hyperbowlee_28
(minor tail protein)

Hyperbowlee_29

Hyperbowlee_30
(lysin A)

Hyperbowlee_31
(lysin B)

Hyperbowlee_32
(holin)

Hyperbowlee_33

Hyperbowlee_34

Hyperbowlee_35
(MRE11 double-strand break endo/exonuclease)

Hyperbowlee_36

Hyperbowlee_37

Hyperbowlee_38

Hyperbowlee_39

Hyperbowlee_40

Hyperbowlee_41
(tyrosine integrase)

Hyperbowlee_42

Hyperbowlee_43
(immunity repressor)

Hyperbowlee_44
(Cro protein)

Hyperbowlee_45
(excise)

Hyperbowlee_46

Hyperbowlee_47

Hyperbowlee_48

Hyperbowlee_49
(MerR-like helix-turn-helix DNA binding domain protein)

Hyperbowlee_50
(WhiB family transcription factor)

Hyperbowlee_51

Hyperbowlee_52

Hyperbowlee_53

Hyperbowlee_54

Hyperbowlee_55

Hyperbowlee_56

Hyperbowlee_57
(DnaE-like DNA polymerase III alpha)

Hyperbowlee_58

Hyperbowlee_59

Hyperbowlee_60

Hyperbowlee_61
(Cas4 family exonuclease)

Hyperbowlee_62

Hyperbowlee_63

Hyperbowlee_64

Hyperbowlee_65

Hyperbowlee_66
(NrdH-like glutaredoxin)

Hyperbowlee_67

Hyperbowlee_68
(HNH endonuclease)

Hyperbowlee_69
(DNA primase/helicase)

Hyperbowlee_70
(RusA-like resolvase)

Hyperbowlee_71

Hyperbowlee_72

Hyperbowlee_73

Hyperbowlee_74

Hyperbowlee_75
(SprT-like protease)

Hyperbowlee_76

Hyperbowlee_77

Hyperbowlee_78

Hyperbowlee_79

Hyperbowlee_80

Hyperbowlee_81

Hyperbowlee_82

Hyperbowlee_83

Hyperbowlee_84
(RtcB-like RNA ligase)

Hyperbowlee_85
(DNA binding protein)

Hyperbowlee_86

Hyperbowlee_87

Hyperbowlee_88

Hyperbowlee_89

Hyperbowlee_90
(HicB-like antitoxin)

Hyperbowlee_91
(HicA-like toxin)

Hyperbowlee_92

Hyperbowlee_93

Hyperbowlee_94

Hyperbowlee_95
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOM818330
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.62E10
Date of SEA Lysate Titering Oct 10, 2014
Pitt Freezer Box# 7
Pitt Freezer Box Grid# F8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload