Mycobacterium phage Topper
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Detailed Information for Phage Topper
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDevin Clark
Year Found2021
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.9847 N, 86.456 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1210
Genome length (bp)59708
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Topper_1

Topper_2

Topper_3

Topper_4

Topper_5 — tRNA
(tRNA-Trp(cca))

Topper_6

Topper_7

Topper_8
(terminase)

Topper_9
(portal protein)

Topper_10
(capsid accessory protein)

Topper_11

Topper_12
(scaffolding protein)

Topper_13
(major capsid protein)

Topper_14
(head-to-tail adaptor)

Topper_15
(head-to-tail stopper)

Topper_16

Topper_17
(tail terminator)

Topper_18
(major tail protein)

Topper_19
(tail assembly chaperone)

Topper_20
(tail assembly chaperone)

Topper_21
(tape measure protein)

Topper_22
(minor tail protein)

Topper_23
(minor tail protein)

Topper_24

Topper_25

Topper_26

Topper_27
(minor tail protein)

Topper_28

Topper_29

Topper_30
(lysin A)

Topper_31
(lysin B)

Topper_32
(holin)

Topper_33

Topper_34

Topper_35
(MRE11 double-strand break endo/exonuclease)

Topper_36
(helix-turn-helix DNA binding domain protein)

Topper_37

Topper_38

Topper_39

Topper_40

Topper_41
(tyrosine integrase)

Topper_42

Topper_43
(immunity repressor)

Topper_44
(Cro protein)

Topper_45
(excise)

Topper_46

Topper_47

Topper_48

Topper_49

Topper_50
(WhiB family transcription factor)

Topper_51

Topper_52

Topper_53

Topper_54

Topper_55

Topper_56

Topper_57
(exonuclease)

Topper_58

Topper_59

Topper_60

Topper_61
(Cas4 family exonuclease)

Topper_62

Topper_63

Topper_64

Topper_65

Topper_66
(NrdH-like glutaredoxin)

Topper_67

Topper_68
(HNH endonuclease)

Topper_69
(DNA primase/helicase)

Topper_70

Topper_71
(RusA-like resolvase)

Topper_72

Topper_73
(MerR-like helix-turn-helix DNA binding domain protein)

Topper_74

Topper_75

Topper_76
(SprT-like protease)

Topper_77

Topper_78

Topper_79

Topper_80

Topper_81
(helix-turn-helix DNA binding domain protein)

Topper_82

Topper_83

Topper_84

Topper_85
(RtcB-like RNA ligase)

Topper_86

Topper_87

Topper_88

Topper_89

Topper_90

Topper_91
(HicB-like antitoxin)

Topper_92
(HicA-like toxin)

Topper_93

Topper_94

Topper_95

Topper_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456339
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.96x10^8
Pitt Freezer Box# 138
Pitt Freezer Box Grid# A7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload