| Detailed Information for Phage Reptar3000 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tiffany Jeanite, Sophia Nhan, Dr. Christy Strong |
| Year Found | 2017 |
| Location Found | Las Vegas, NV USA |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.027427 N, 115.207002 W Map |
| Discovery Notes | The soil sample from where the phages originated was obtained from the roots of an unidentified red plant.
Time and Date of Extraction: 9:24am; 09/04/2017
Temperature of Area of Extraction: 83F Humidity: 31%
Wind speed: N 7 mph
Cloud cover: Sunny |
| Naming Notes | Both members of the group were fans of the Rugrats when they were children and decided to name the phage Reptar. The 3000 part was added to make it sound more futuristic. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 19, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 946 |
| Genome length (bp) | 54601 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGCGGCCAT |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | In GenBank |
| Plaque Notes | Pinpoint shape
Turbid borders
About 1 mm in diameter with a .1mm Halo surrounding it |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH926058 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
52 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
