Mycobacterium phage Reptar3000
Reptar3000 Plaque Picture
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Detailed Information for Phage Reptar3000
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTiffany Jeanite, Sophia Nhan, Dr. Christy Strong
Year Found2017
Location FoundLas Vegas, NV USA
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.027427 N, 115.207002 W Map
Discovery NotesThe soil sample from where the phages originated was obtained from the roots of an unidentified red plant.

Time and Date of Extraction: 9:24am; 09/04/2017
Temperature of Area of Extraction: 83F Humidity: 31%
Wind speed: N 7 mph
Cloud cover: Sunny
Naming NotesBoth members of the group were fans of the Rugrats when they were children and decided to name the phage Reptar. The 3000 part was added to make it sound more futuristic.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 19, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage946
Genome length (bp)54601
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content67.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of Nevada Las Vegas
Annotation StatusIn GenBank
Plaque NotesPinpoint shape
Turbid borders
About 1 mm in diameter with a .1mm Halo surrounding it
MorphotypeSiphoviridae
Number of Genes88
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Reptar3000_1

Reptar3000_2

Reptar3000_3

Reptar3000_4

Reptar3000_5

Reptar3000_6

Reptar3000_7

Reptar3000_8

Reptar3000_9
(terminase)

Reptar3000_10
(portal protein)

Reptar3000_11
(MuF-like minor capsid protein)

Reptar3000_12

Reptar3000_13
(scaffolding protein)

Reptar3000_14
(major capsid protein)

Reptar3000_15
(head-to-tail adaptor)

Reptar3000_16
(head-to-tail stopper)

Reptar3000_17

Reptar3000_18
(tail terminator)

Reptar3000_19
(major tail protein)

Reptar3000_20
(tail assembly chaperone)

Reptar3000_21
(tail assembly chaperone)

Reptar3000_22
(tape measure protein)

Reptar3000_23
(minor tail protein)

Reptar3000_24
(minor tail protein)

Reptar3000_25
(minor tail protein)

Reptar3000_26
(minor tail protein)

Reptar3000_27
(minor tail protein)

Reptar3000_28
(minor tail protein)

Reptar3000_29
(minor tail protein)

Reptar3000_30

Reptar3000_31
(lysin A)

Reptar3000_32
(lysin B)

Reptar3000_33
(holin)

Reptar3000_34

Reptar3000_35

Reptar3000_36
(MRE11 double-strand break endo/exonuclease)

Reptar3000_37

Reptar3000_38 — tRNA

Reptar3000_39
(LamD-like)

Reptar3000_40

Reptar3000_41

Reptar3000_42

Reptar3000_43
(tyrosine integrase)

Reptar3000_44

Reptar3000_45
(immunity repressor)

Reptar3000_46
(Cro protein)

Reptar3000_47
(excise)

Reptar3000_48

Reptar3000_49

Reptar3000_50

Reptar3000_51

Reptar3000_52

Reptar3000_53

Reptar3000_54

Reptar3000_55

Reptar3000_56

Reptar3000_57
(HicA-like toxin)

Reptar3000_58

Reptar3000_59

Reptar3000_60

Reptar3000_61
(Cas4 family exonuclease)

Reptar3000_62

Reptar3000_63

Reptar3000_64

Reptar3000_65

Reptar3000_66

Reptar3000_67
(NrdH-like glutaredoxin)

Reptar3000_68
(methyltransferase)

Reptar3000_69

Reptar3000_70
(HNH endonuclease)

Reptar3000_71
(DNA primase)

Reptar3000_72

Reptar3000_73
(HNH endonuclease)

Reptar3000_74

Reptar3000_75

Reptar3000_76

Reptar3000_77

Reptar3000_78
(SprT-like protease)

Reptar3000_79

Reptar3000_80

Reptar3000_81

Reptar3000_82

Reptar3000_83

Reptar3000_84

Reptar3000_85

Reptar3000_86

Reptar3000_87

Reptar3000_88
(HNH endonuclease)

Reptar3000_89
(methyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH926058
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 52
Pitt Freezer Box Grid# D3
Available Files
EM PictureDownload
GenBank File for PhameratorDownload