Mycobacterium phage Hammy
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Detailed Information for Phage Hammy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGabriella Reyes
Year Found2015
Location FoundSlidell, LA USA
Finding InstitutionUniversity of Southern Mississippi
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates30.291389 N, 89.723611 W Map
Discovery NotesFrom the bank of Cross Gates neighborhood pond, also located behind an Autozone; compacted, moist soil, dug about 2 inches down to get sample; approximate air temperature: 90F
Naming NotesThroughout these lab experiments, my partner and I were always hungry, and we thought that fresh agar plates smelled like ham, so we named our phage “Hammy”
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2914
Genome length (bp)61812
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content67.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK6
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesOur plaques were clear and measured about 3mm in diameter
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Hammy_1

Hammy_2

Hammy_3

Hammy_5

Hammy_6

Hammy_7
(terminase)

Hammy_8
(portal protein)

Hammy_9
(MuF-like minor capsid protein)

Hammy_10

Hammy_11
(scaffolding protein)

Hammy_12
(major capsid protein)

Hammy_13
(head-to-tail adaptor)

Hammy_14
(head-to-tail stopper)

Hammy_15

Hammy_16
(tail terminator)

Hammy_17
(major tail protein)

Hammy_18
(tail assembly chaperone)

Hammy_19
(tail assembly chaperone)

Hammy_20
(tape measure protein)

Hammy_21
(minor tail protein)

Hammy_22
(minor tail protein)

Hammy_23
(minor tail protein)

Hammy_24
(minor tail protein)

Hammy_25
(minor tail protein)

Hammy_26
(minor tail protein)

Hammy_27

Hammy_28

Hammy_29
(lysin A)

Hammy_30
(lysin B)

Hammy_31
(holin)

Hammy_32

Hammy_33

Hammy_34
(MRE11 double-strand break endo/exonuclease)

Hammy_35

Hammy_36

Hammy_37

Hammy_38

Hammy_39
(queuine tRNA-ribosyltransferase)

Hammy_40

Hammy_41
(helix-turn-helix DNA binding protein)

Hammy_42
(tyrosine integrase)

Hammy_43

Hammy_44

Hammy_45
(immunity repressor)

Hammy_46
(Cro protein)

Hammy_47
(excise)

Hammy_48

Hammy_49

Hammy_50

Hammy_51

Hammy_52

Hammy_53
(WhiB family transcription factor)

Hammy_54

Hammy_55

Hammy_56

Hammy_57

Hammy_58
(DNA polymerase II exonuclease)

Hammy_59

Hammy_60
(Cas4 family exonuclease)

Hammy_61

Hammy_62

Hammy_63

Hammy_64

Hammy_65

Hammy_66
(NrdH-like glutaredoxin)

Hammy_67
(HNH endonuclease)

Hammy_68
(DNA primase/helicase)

Hammy_69
(RusA-like resolvase)

Hammy_70

Hammy_71

Hammy_72

Hammy_73

Hammy_74

Hammy_75

Hammy_76

Hammy_77

Hammy_78

Hammy_79

Hammy_80

Hammy_81

Hammy_82

Hammy_83
(RtcB-like RNA ligase)

Hammy_84

Hammy_85

Hammy_86

Hammy_87

Hammy_88

Hammy_89

Hammy_90

Hammy_91

Hammy_92

Hammy_93

Hammy_94

Hammy_95
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKY087993
Refseq NumberNone yet
Published in PaperAnders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.59 x 10^9
Date of SEA Lysate Titering Nov 13, 2015
Pitt Freezer Box# 18
Pitt Freezer Box Grid# B6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload