The Actinobacteriophage Database

Mycobacterium phage BEEST

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Detailed Information for Phage BEEST
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Lisa Deng and Patrick Ng
Year Found	2008
Location Found	St Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	38.650249 N, 90.316691 W Map
Discovery Notes	The soil was collected from a site that is used as a municipal mulch pile. Soil depth 1cm at 70 degrees.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	May 31, 2011
Genome length (bp)	59906
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CTCGTAGGCAT
GC Content	66.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) Capricorn (K1) CaseJules (K1) CheetoDust (K1) Chris (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jayhawk (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Llorens (K1) Lunahalos (K1) MarkPhew (K1) Mdavu (K1) MeaningOfLife (K1) Megsy (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) TiniBug (K1) Tiri (K1) Topper (K1) TreyKay (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zabiza (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) Strobilo (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) MacnCheese (K3) Pharb (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Chancellor (K4) Cheetobro (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) Agent47 (K5) AlleyCat (K5) Collard (K5) Dadosky (K5) Edugator (K5) Feyre (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) Miryou (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) TClif (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7) Boilgate (K8)
Annotating Institution	Washington University in St. Louis
Annotation Status	In GenBank
Plaque Notes	3mm/turbid and haloed/ incubation 24 hours at 37 degrees Celsius
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View BEEST_1 BEEST_2 BEEST_3 BEEST_4 BEEST_6 BEEST_7 BEEST_8 (terminase) BEEST_9 (portal protein) BEEST_10 (MuF-like minor capsid protein) BEEST_11 BEEST_12 (scaffolding protein) BEEST_13 (major capsid protein) BEEST_14 (head-to-tail adaptor) BEEST_15 (head-to-tail stopper) BEEST_16 BEEST_17 (tail terminator) BEEST_18 BEEST_19 BEEST_20 BEEST_21 (tape measure protein) BEEST_22 (minor tail protein) BEEST_23 (minor tail protein) BEEST_24 (minor tail protein) BEEST_25 (minor tail protein) BEEST_26 (minor tail protein) BEEST_27 (minor tail protein) BEEST_28 (minor tail protein) BEEST_29 BEEST_30 (lysin A) BEEST_31 (lysin B) BEEST_32 (holin) BEEST_33 BEEST_34 BEEST_35 (MRE11 double-strand break endo/exonuclease) BEEST_36 BEEST_37 BEEST_38 BEEST_39 BEEST_40 BEEST_41 (tyrosine integrase) BEEST_42 BEEST_43 (immunity repressor) BEEST_44 (helix-turn-helix DNA binding protein) BEEST_45 (excise) BEEST_46 BEEST_47 BEEST_48 BEEST_49 (MerR-like helix-turn-helix DNA binding domain protein) BEEST_50 (WhiB family transcription factor) BEEST_51 BEEST_52 BEEST_53 BEEST_54 BEEST_55 BEEST_56 BEEST_57 (DnaE-like DNA polymerase III) BEEST_58 BEEST_59 BEEST_60 BEEST_61 (Cas4 family endonuclease) BEEST_62 BEEST_63 BEEST_64 BEEST_65 BEEST_66 (NrdH-like glutaredoxin) BEEST_67 BEEST_68 (HNH endonuclease) BEEST_69 (DNA primase/helicase) BEEST_70 BEEST_71 (RusA-like resolvase) BEEST_72 BEEST_73 BEEST_74 BEEST_75 BEEST_76 (SprT-like protease) BEEST_77 BEEST_78 BEEST_79 BEEST_80 BEEST_81 BEEST_82 BEEST_83 BEEST_84 BEEST_85 (RtcB-like RNA ligase) BEEST_86 BEEST_87 BEEST_88 BEEST_89 BEEST_90 BEEST_91 (HicB-like antitoxin) BEEST_92 (HicA-like toxin) BEEST_93 BEEST_94 BEEST_95 BEEST_96 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH509444
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Lysate Titer	3.2e10
Pitt Freezer Box#	10
Pitt Freezer Box Grid#	9
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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