| Detailed Information for Phage LeMond |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | DAJM, Sharkeisha |
| Found By | Jack Murphy and David Ahern |
| Year Found | 2014 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 47.665923 N, 117.403924 W Map |
| Discovery Notes | Soil taken at the base of a willow tree, next to a swampy lake. Located at the base of a hill near Hughes Hall on Gonzaga's campus. Soil appeared to be dry. Some small rocks and dry grass in sample. |
| Naming Notes | Named in honor of the great cyclist, Greg LeMond. (Please note this lysate was named "Sharkeisha" at the home institution and the students know it by that name. It was changed to LeMond later to satisfy PhagesDB naming rules.) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 107 |
| Genome length (bp) | 62515 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appeared cloudy and turbid. Plaques had a diameter of 2.0-2.5 millimeters. All plaques had a halo of approximately 0.3 millimeters. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH910038 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
16 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
