The Actinobacteriophage Database

Mycobacterium phage Amelie

Locally BLAST this genome

Run GeneMarkS

Run GeneMark
(smeg)

Run GeneMark
(TB_H37Rv)

Know something about this phage that we don't? Modify its data.

Detailed Information for Phage Amelie
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Delfina Grinspan
Year Found	2012
Location Found	St. Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	38.647778 N, 90.299722 W Map
Discovery Notes	Amelie was the 500th sequenced Mycobacteriophage on PhagesDB!
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jun 4, 2013
Sequencing Facility	The McDonnell Genome Institute at Washington University
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	232
Sanger Finishing Reactions	3
Genome length (bp)	56439
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CTCGGGGGCAT
GC Content	67.1%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) Capricorn (K1) CaseJules (K1) CheetoDust (K1) Chris (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jayhawk (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Llorens (K1) Lunahalos (K1) MarkPhew (K1) Mdavu (K1) MeaningOfLife (K1) Megsy (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) TiniBug (K1) Tiri (K1) Topper (K1) TreyKay (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zabiza (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) Strobilo (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) MacnCheese (K3) Pharb (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Chancellor (K4) Cheetobro (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) Agent47 (K5) AlleyCat (K5) Collard (K5) Dadosky (K5) Edugator (K5) Feyre (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) Miryou (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) TClif (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7) Boilgate (K8)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Rather small plaques. Not complete clarity.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Amelie_1 Amelie_2 Amelie_3 Amelie_4 (terminase) Amelie_5 (portal protein) Amelie_6 (MuF-like minor capsid protein) Amelie_7 Amelie_8 (scaffolding protein) Amelie_9 (major capsid protein) Amelie_10 (head-to-tail adaptor) Amelie_11 (head-to-tail stopper) Amelie_12 Amelie_13 (tail terminator) Amelie_14 Amelie_15 Amelie_16 Amelie_17 (tape measure protein) Amelie_18 (minor tail protein) Amelie_19 (minor tail protein) Amelie_20 (minor tail protein) Amelie_21 (minor tail protein) Amelie_22 (minor tail protein) Amelie_23 (minor tail protein) Amelie_24 (minor tail protein) Amelie_25 Amelie_26 (lysin A) Amelie_27 (lysin B) Amelie_28 (holin) Amelie_29 Amelie_30 Amelie_31 Amelie_32 Amelie_33 Amelie_34 Amelie_35 (queuine tRNA-ribosyltransferase) Amelie_36 (tyrosine integrase) Amelie_37 Amelie_38 (immunity repressor) Amelie_39 (Cro protein) Amelie_40 (excise) Amelie_41 Amelie_42 Amelie_43 Amelie_44 Amelie_45 Amelie_46 (WhiB family transcription factor) Amelie_47 Amelie_48 Amelie_49 (DnaE-like DNA polymerase III) Amelie_50 Amelie_51 Amelie_52 Amelie_53 Amelie_54 Amelie_55 (NrdH-like glutaredoxin) Amelie_56 Amelie_57 (DNA primase/helicase) Amelie_58 (RusA-like resolvase) Amelie_59 Amelie_60 Amelie_61 Amelie_62 Amelie_63 Amelie_64 Amelie_65 Amelie_66 Amelie_67 (RtcB-like RNA ligase) Amelie_68 Amelie_69 Amelie_70 Amelie_71 Amelie_72 Amelie_73 Amelie_74 Amelie_75 Amelie_76 Amelie_77 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KX808132
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2012WUSLAmelieKH
SEA Lysate Titer	5.78E9
Date of SEA Lysate Titering	Oct 9, 2012
Pitt Freezer Box#	42
Pitt Freezer Box Grid#	2
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

Click to View

Click to View