Mycobacterium phage Amelie
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Detailed Information for Phage Amelie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDelfina Grinspan
Year Found2012
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.647778 N, 90.299722 W Map
Discovery NotesAmelie was the 500th sequenced Mycobacteriophage on PhagesDB!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 4, 2013
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage232
Sanger Finishing Reactions3
Genome length (bp)56439
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGGGGGCAT
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Capricorn (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Inky (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Spock (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesRather small plaques. Not complete clarity.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Amelie_1

Amelie_2

Amelie_3
(terminase small subunit)

Amelie_4
(terminase large subunit)

Amelie_5
(portal protein)

Amelie_6
(capsid maturation protease)

Amelie_7

Amelie_8
(scaffolding protein)

Amelie_9
(major capsid protein)

Amelie_10
(head-to-tail connector protein)

Amelie_11
(head-to-tail connector protein)

Amelie_12
(head-to-tail connector protein)

Amelie_13
(head-to-tail connector protein)

Amelie_14
(major tail protein)

Amelie_15
(tail assembly chaperone protein)

Amelie_16
(tail assembly chaperone protein)

Amelie_17
(tape measure protein)

Amelie_18
(minor tail protein)

Amelie_19
(minor tail protein)

Amelie_20
(minor tail protein)

Amelie_21
(minor tail protein)

Amelie_22
(minor tail protein)

Amelie_23
(minor tail protein)

Amelie_24
(minor tail protein)

Amelie_25

Amelie_26
(lysin A)

Amelie_27
(lysin B)

Amelie_28
(holin)

Amelie_29

Amelie_30

Amelie_31

Amelie_32

Amelie_33

Amelie_34

Amelie_35
(queuine tRNA-ribosyltransferase)

Amelie_36
(integrase)

Amelie_37

Amelie_38
(immunity repressor)

Amelie_39
(helix-turn-helix DNA binding protein)

Amelie_40
(helix-turn-helix DNA binding protein)

Amelie_41

Amelie_42

Amelie_43

Amelie_44

Amelie_45

Amelie_46
(WhiB family transcription factor)

Amelie_47

Amelie_48

Amelie_49
(DNA polymerase III subunit)

Amelie_50

Amelie_51

Amelie_52

Amelie_53

Amelie_54

Amelie_55
(glutaredoxin)

Amelie_56

Amelie_57
(DNA primase)

Amelie_58
(resolvase)

Amelie_59

Amelie_60

Amelie_61

Amelie_62

Amelie_63

Amelie_64

Amelie_65

Amelie_66

Amelie_67
(RNA ligase)

Amelie_68

Amelie_69

Amelie_70

Amelie_71

Amelie_72

Amelie_73

Amelie_74

Amelie_75

Amelie_76

Amelie_77
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX808132
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2012WUSLAmelieKH
SEA Lysate Titer 5.78E9
Date of SEA Lysate Titering Oct 9, 2012
Pitt Freezer Box# 42
Pitt Freezer Box Grid# 2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload