| Detailed Information for Phage Anaya |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ariangela Joy Davis |
| Year Found | 2009 |
| Location Found | Grand Rapids, MI USA |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.933022 N, 85.587226 W Map |
| Discovery Notes | Outside the side door of Noordewier Hall, when coming out of the door, in the grass to your right, around the first inground sprinkler head |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 17, 2011 |
| Sequencing Facility | Joint Genomics Institute |
| Genome length (bp) | 60835 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques were…..mm. They typically had two different sizes, a larger and one quite a bit smaller. The plaques were clear in the middle and there were rings around the edges, like a halo. They typically took 48h to grow and the plates were incubated at 30°C. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | JF704106 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2009CALVanayaAJD |
| SEA Lysate Titer |
1e9 |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
