Mycobacterium phage Asayake
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Asayake
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByYong Chul Yoon
Year Found2014
Location FoundHOLLAND, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates42.788333 N, 86.100278 W Map
Discovery NotesAsayake was discovered from a soil collected from the pavement near Martha Miller Multicultural and Communication Center at Hope College. The temperature was about 82°F at the time of discovery and the soil was humid.
Naming NotesAsayke means red glow in the morning in Japanese. It is also the name of a song that I listen to whenever I work on my phage lab stuff.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 1, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage269
Genome length (bp)59905
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesAsayke does not grow at 42°C. From the observations made in the previous papers, it is likely that the Asayake grows at a temperature above 42°C.

Also, from the bacteria collected from the mesa, it was confirmed that Asayake produces stable lysoges, meaning Asayke is temperate.
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUniversity of Massachusetts Amherst
Annotation StatusIn GenBank
Plaque NotesAsayke is a temperate phage that grows to about 0.25cm in 24hrs and 0.41cm in 48 hours. Its plaques are circular and have slightly bull's eye-like morphology.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Asayake_1

Asayake_2

Asayake_3
(ATPase domain protein)

Asayake_4

Asayake_6

Asayake_7

Asayake_8
(terminase)

Asayake_9
(portal protein)

Asayake_10
(capsid maturation protease)

Asayake_11

Asayake_12
(scaffolding protein)

Asayake_13
(major capsid protein)

Asayake_14
(head-to-tail adaptor)

Asayake_15
(head-to-tail stopper)

Asayake_16

Asayake_17
(tail terminator)

Asayake_18
(major tail protein)

Asayake_19
(tail assembly chaperone)

Asayake_20
(tail assembly chaperone)

Asayake_21
(tape measure protein)

Asayake_22
(minor tail protein)

Asayake_23
(minor tail protein)

Asayake_24

Asayake_25
(minor tail protein)

Asayake_26

Asayake_27
(minor tail protein)

Asayake_28
(minor tail protein)

Asayake_29

Asayake_30
(lysin A)

Asayake_31
(lysin B)

Asayake_32
(holin)

Asayake_33

Asayake_34

Asayake_35
(exonuclease)

Asayake_36
(helix-turn-helix DNA binding domain protein)

Asayake_37

Asayake_38

Asayake_39

Asayake_40

Asayake_41
(tyrosine integrase)

Asayake_42

Asayake_43
(immunity repressor)

Asayake_44
(helix-turn-helix DNA binding protein)

Asayake_45
(excise)

Asayake_46

Asayake_47

Asayake_48

Asayake_49
(helix-turn-helix DNA binding domain protein)

Asayake_50
(WhiB family transcription factor)

Asayake_51

Asayake_52

Asayake_53

Asayake_54

Asayake_55

Asayake_56

Asayake_57
(dnaq-like DNA polymerase iii subunit)

Asayake_58

Asayake_59

Asayake_60

Asayake_61
(exonuclease)

Asayake_62

Asayake_63

Asayake_64

Asayake_65

Asayake_66
(NrdH glutaredoxin)

Asayake_67

Asayake_68
(HNH endonuclease)

Asayake_69
(DNA primase)

Asayake_70

Asayake_71
(RusA-like resolvase)

Asayake_72

Asayake_73

Asayake_74

Asayake_75

Asayake_76
(SprT-like protease)

Asayake_77

Asayake_78

Asayake_79
(helix-turn-helix DNA binding domain protein)

Asayake_80

Asayake_81

Asayake_82

Asayake_83

Asayake_84

Asayake_85
(RtcB-like RNA ligase)

Asayake_86

Asayake_87

Asayake_88

Asayake_89
(helix-turn-helix DNA binding domain protein)

Asayake_90

Asayake_91
(HicB-like antitoxin)

Asayake_92
(HicA-like toxin)

Asayake_93

Asayake_94

Asayake_95

Asayake_96

Asayake_97
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW712723
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.66e10
Pitt Freezer Box# 7
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
GenBank File for PhameratorDownload