Mycobacterium phage Veliki
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Detailed Information for Phage Veliki
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaroline Degraw and Sayre Weast
Year Found2015
Location FoundHattiesburg, MS USA
Finding InstitutionUniversity of Southern Mississippi
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates31.998056 N, 89.3325 W Map
Discovery NotesFlowerbed outside Century Park dorm, flower bed receives some sunlight, surface level soil, 87°F
Naming NotesVeliki means "large" in Bosnian, and our plaques were fairly large
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 14, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage2066
Genome length (bp)59734
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesOur plaques had a 5 mm diameter and were clear
MorphotypeSiphoviridae
Number of Genes96
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Veliki_1

Veliki_2

Veliki_3
(DNA binding protein)

Veliki_4

Veliki_5 — tRNA
(tRNA-Trp(cca))

Veliki_6

Veliki_7

Veliki_8
(terminase)

Veliki_9
(portal protein)

Veliki_10
(MuF-like minor capsid protein)

Veliki_11

Veliki_12
(scaffolding protein)

Veliki_13
(major capsid protein)

Veliki_14
(head-to-tail adaptor)

Veliki_15
(head-to-tail stopper)

Veliki_16

Veliki_17
(tail terminator)

Veliki_18

Veliki_19

Veliki_20

Veliki_21
(tape measure protein)

Veliki_22
(minor tail protein)

Veliki_23
(minor tail protein)

Veliki_24
(minor tail protein)

Veliki_25
(minor tail protein)

Veliki_26

Veliki_27
(minor tail protein)

Veliki_28
(minor tail protein)

Veliki_29

Veliki_30
(lysin A)

Veliki_31
(lysin B)

Veliki_32
(holin)

Veliki_33

Veliki_34

Veliki_35
(MRE11 double-strand break endo/exonuclease)

Veliki_36

Veliki_37

Veliki_38

Veliki_39

Veliki_40

Veliki_41
(tyrosine integrase)

Veliki_42

Veliki_43
(immunity repressor)

Veliki_44
(helix-turn-helix DNA binding protein)

Veliki_45
(excise)

Veliki_46

Veliki_47

Veliki_48

Veliki_49

Veliki_50
(MerR-like helix-turn-helix DNA binding domain protein)

Veliki_51
(WhiB family transcription factor)

Veliki_52

Veliki_53

Veliki_54

Veliki_55

Veliki_56

Veliki_57

Veliki_58
(DnaE-like DNA polymerase III)

Veliki_59

Veliki_60

Veliki_61

Veliki_62
(Cas4 family endonuclease)

Veliki_63

Veliki_64

Veliki_65

Veliki_66

Veliki_67

Veliki_68
(NrdH-like glutaredoxin)

Veliki_69
(HNH endonuclease)

Veliki_70
(DNA primase/helicase)

Veliki_71

Veliki_72
(RusA-like resolvase)

Veliki_73

Veliki_74

Veliki_75

Veliki_76

Veliki_77
(SprT-like protease)

Veliki_78

Veliki_79

Veliki_80

Veliki_81

Veliki_82

Veliki_83

Veliki_84

Veliki_85

Veliki_86
(RtcB-like RNA ligase)

Veliki_87

Veliki_88

Veliki_89

Veliki_90

Veliki_91

Veliki_92

Veliki_93

Veliki_94

Veliki_95

Veliki_96

Veliki_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN234205
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.23 x 10^10
Date of SEA Lysate Titering Oct 26, 2015
Pitt Freezer Box# 18
Pitt Freezer Box Grid# B10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload