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Mycobacterium phage Biglebops
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Detailed Information for Phage Biglebops
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBraden Bigelow
Year Found2016
Location FoundLondon, KY USA
Finding InstitutionSomerset Community College
ProgramKentucky Biomedical Infrastructure Network Small Genomes Discovery Program
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.129 N, 84.0833 W Map
Discovery Notes12 inches below ground in garden
He was born in a garden after a really big snow break
Found in some mushy wet soil

Naming NotesI named him this because his name is amazing
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 23, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage270
Genome length (bp)56454
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGGGGGCAT
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionSomerset Community College
Annotation StatusIn GenBank
Plaque NotesClear Pretty circular
Very tiny had to have a lot to fill my plate
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Biglebops_1

Biglebops_2

Biglebops_3

Biglebops_4
(terminase)

Biglebops_5
(portal protein)

Biglebops_6
(MuF-like minor capsid protein)

Biglebops_7

Biglebops_8
(scaffolding protein)

Biglebops_9
(major capsid protein)

Biglebops_10
(head-to-tail adaptor)

Biglebops_11
(head-to-tail stopper)

Biglebops_12

Biglebops_13
(tail terminator)

Biglebops_14

Biglebops_15

Biglebops_16

Biglebops_17
(tape measure protein)

Biglebops_18
(minor tail protein)

Biglebops_19
(minor tail protein)

Biglebops_20
(minor tail protein)

Biglebops_21
(minor tail protein)

Biglebops_22
(minor tail protein)

Biglebops_23
(minor tail protein)

Biglebops_24
(minor tail protein)

Biglebops_25

Biglebops_26
(lysin A)

Biglebops_27
(lysin B)

Biglebops_28
(holin)

Biglebops_29

Biglebops_30

Biglebops_31
(MRE11 double-strand break endo/exonuclease)

Biglebops_32

Biglebops_33

Biglebops_34

Biglebops_35
(queuine tRNA-ribosyltransferase)

Biglebops_36
(tyrosine integrase)

Biglebops_37

Biglebops_38
(immunity repressor)

Biglebops_39
(Cro protein)

Biglebops_40
(excise)

Biglebops_41

Biglebops_42

Biglebops_43

Biglebops_44

Biglebops_45
(MerR-like helix-turn-helix DNA binding domain protein)

Biglebops_46
(WhiB family transcription factor)

Biglebops_47

Biglebops_48

Biglebops_49
(DnaE-like DNA polymerase III)

Biglebops_50
(Cas4 family endonuclease)

Biglebops_51

Biglebops_52

Biglebops_53

Biglebops_54

Biglebops_55
(NrdH-like glutaredoxin)

Biglebops_56
(HNH endonuclease)

Biglebops_57
(DNA primase/helicase)

Biglebops_58
(RusA-like resolvase)

Biglebops_59

Biglebops_60

Biglebops_61

Biglebops_62

Biglebops_63

Biglebops_64

Biglebops_65

Biglebops_66
(polynucleotide kinase)

Biglebops_67
(RtcB-like RNA ligase)

Biglebops_68

Biglebops_69

Biglebops_70

Biglebops_71

Biglebops_72
(HicB-like antitoxin)

Biglebops_73
(HicA-like toxin)

Biglebops_74

Biglebops_75

Biglebops_76

Biglebops_77
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399770
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.4x10^11 pfu/mL
Date of SEA Lysate Titering Apr 28, 2016
Pitt Freezer Box# 28
Pitt Freezer Box Grid# B1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload