Mycobacterium phage Eponine
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Detailed Information for Phage Eponine
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBrielle Paul
Year Found2018
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.0 N, 75.0 W Map
Discovery Notes-Collected outside Richards House at Lehigh University.

-Soil was a little dry, with some evidence of organic matter. Soil was dark brown in color.

-Soil was collected approx. a couple of inches below the surface.

-Date collected: January 21, 2018
Naming NotesEponine is the name of my favorite character in the Broadway musical, Les Miserables.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 23, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1150
Genome length (bp)58678
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Inky (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque Notes-Small clear plaques, about 1-2 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Eponine_1

Eponine_2

Eponine_3

Eponine_4

Eponine_5

Eponine_6

Eponine_7

Eponine_8

Eponine_9
(terminase)

Eponine_10
(portal protein)

Eponine_11
(capsid maturation protease)

Eponine_12

Eponine_13
(scaffolding protein)

Eponine_14
(major capsid protein)

Eponine_15
(head-to-tail adaptor)

Eponine_16
(head-to-tail stopper)

Eponine_17

Eponine_18
(tail terminator)

Eponine_19
(major tail protein)

Eponine_20
(tail assembly chaperone)

Eponine_21
(tail assembly chaperone)

Eponine_22
(tape measure protein)

Eponine_23
(minor tail protein)

Eponine_24
(minor tail protein)

Eponine_25
(minor tail protein)

Eponine_26
(minor tail protein)

Eponine_27
(minor tail protein)

Eponine_28
(minor tail protein)

Eponine_29
(minor tail protein)

Eponine_30

Eponine_31

Eponine_32

Eponine_33
(lysin A)

Eponine_34
(lysin B)

Eponine_35
(holin)

Eponine_36

Eponine_37

Eponine_38
(phosphoesterase)

Eponine_39

Eponine_41
(helix-turn-helix DNA binding domain protein)

Eponine_42

Eponine_43

Eponine_44

Eponine_45

Eponine_46

Eponine_47
(tyrosine integrase)

Eponine_48

Eponine_49
(immunity repressor)

Eponine_50
(Cro protein)

Eponine_51
(excise)

Eponine_52

Eponine_53

Eponine_54

Eponine_55

Eponine_56

Eponine_57

Eponine_58

Eponine_59

Eponine_60

Eponine_61
(HicA-like toxin)

Eponine_62

Eponine_63

Eponine_64
(DNA polymerase III subunit)

Eponine_65

Eponine_66
(exonuclease)

Eponine_67

Eponine_68

Eponine_69

Eponine_70

Eponine_71

Eponine_72
(NrdH-like glutaredoxin)

Eponine_73
(DNA methylase)

Eponine_74

Eponine_75
(HNH endonuclease)

Eponine_76
(DNA primase)

Eponine_77

Eponine_78
(HNH endonuclease)

Eponine_79

Eponine_80
(helix-turn-helix DNA binding domain protein)

Eponine_81

Eponine_82

Eponine_83
(SprT-like protease)

Eponine_84

Eponine_85

Eponine_86

Eponine_87

Eponine_88

Eponine_89

Eponine_90

Eponine_91

Eponine_92

Eponine_93

Eponine_94

Eponine_95

Eponine_96
(HNH endonuclease)

Eponine_97
(methyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN945904
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.0X10^10
Pitt Freezer Box# 84
Pitt Freezer Box Grid# F1
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload