Mycobacterium phage KiSi
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Detailed Information for Phage KiSi
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMonica Kinder & Elisabeth Sitio
Year Found2014
Location FoundSpokane, WA United States of America
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates47.667074 N, 117.402995 W Map
Discovery NotesSoil sample was collected from a puddle near the front of Hughes building at Gonzaga University in Spokane Washington.
Naming NotesMy lab partner and I combined the first two letters of our last names to make the name of our phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage333
Genome length (bp)62558
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content68.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TrippyB (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

Inky (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

NimNO (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Spock (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesThe plaques were 3mm in diameter and cloudy.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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KiSi_1

KiSi_2

KiSi_3

KiSi_5

KiSi_6
(terminase small subunit)

KiSi_7
(terminase large subunit)

KiSi_8
(portal protein)

KiSi_9
(capsid maturation protease)

KiSi_10

KiSi_11
(scaffolding protein)

KiSi_12
(major capsid protein)

KiSi_13
(head-to-tail connector complex protein)

KiSi_14
(head-to-tail connector complex protein)

KiSi_15

KiSi_16
(head-to-tail connector complex protein)

KiSi_17
(major tail protein)

KiSi_18
(tail assembly chaperone)

KiSi_19
(tail assembly chaperone)

KiSi_20
(tape measure protein)

KiSi_21
(minor tail protein)

KiSi_22
(minor tail protein)

KiSi_23
(minor tail protein)

KiSi_24
(minor tail protein)

KiSi_25
(minor tail protein)

KiSi_26
(minor tail protein)

KiSi_27
(minor tail protein)

KiSi_28

KiSi_29
(lysin A)

KiSi_30
(lysin B)

KiSi_31
(holin)

KiSi_32

KiSi_33

KiSi_34

KiSi_35
(nuclease)

KiSi_36

KiSi_37
(helix-turn-helix DNA binding domain protein)

KiSi_38

KiSi_39

KiSi_40

KiSi_41

KiSi_42

KiSi_43

KiSi_44
(queuine tRNA-ribosyltransferase)

KiSi_45
(integrase)

KiSi_46
(membrane protein)

KiSi_47
(immunity repressor)

KiSi_48
(Cro protein)

KiSi_49
(excise)

KiSi_50

KiSi_51

KiSi_52

KiSi_53

KiSi_54
(helix-turn-helix DNA binding domain protein)

KiSi_55
(WhiB family transcription factor)

KiSi_56

KiSi_57

KiSi_58

KiSi_59

KiSi_60

KiSi_61
(DnaQ-like exonuclease)

KiSi_62

KiSi_63

KiSi_64

KiSi_65
(exonuclease)

KiSi_66

KiSi_67

KiSi_68

KiSi_69

KiSi_70

KiSi_71
(NrdH-like glutaredoxin)

KiSi_72
(HNH endonuclease)

KiSi_73
(DNA primase/polymerase)

KiSi_74
(rusa-like resolvase)

KiSi_75

KiSi_76
(helix-turn-helix DNA binding domain protein)

KiSi_77

KiSi_78

KiSi_79
(SprT-like protease)

KiSi_80

KiSi_81
(helix-turn-helix DNA binding domain protein)

KiSi_82

KiSi_83
(helix-turn-helix DNA binding domain protein)

KiSi_84

KiSi_85

KiSi_86

KiSi_87
(RtcB-like RNA ligase)

KiSi_88

KiSi_89

KiSi_90

KiSi_91

KiSi_92

KiSi_93

KiSi_94

KiSi_95

KiSi_96
(HicB-like antitoxin)

KiSi_97
(HicA-like toxin)

KiSi_98
(esterase)

KiSi_99

KiSi_100

KiSi_101

KiSi_102

KiSi_103

KiSi_104
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK376955
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.15x10^10 pfu/mL
Pitt Freezer Box# 16
Pitt Freezer Box Grid# G3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload