Mycobacterium phage Ruthiejr
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Detailed Information for Phage Ruthiejr
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJennifer LaRoche
Year Found2008
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.7842 N, 86.09175 W Map
Discovery NotesFrom topsoil in a rural area, on the bank of a swampy pond, next to a walking bridge. 76° F

-Two different plaque morphologies were found (A and B)
-A had a diameter of 2mm
-B had a diameter of 7mm
-Plaque A (2008HOPEruthiejrJRL) was followed up
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 9, 2019
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage401
Genome length (bp)57858
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

Topper (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionWorcester Polytechnic Institute
Annotation StatusIn GenBank
Plaque Notes-bulls eye (haloed)
-2-3mm in diameter
-incubation time: checked at 24 and 48 hours
-incubated at 37˚ C
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Ruthiejr_1

Ruthiejr_2

Ruthiejr_3

Ruthiejr_4

Ruthiejr_5

Ruthiejr_6

Ruthiejr_7

Ruthiejr_8
(terminase)

Ruthiejr_9
(portal protein)

Ruthiejr_10

Ruthiejr_11

Ruthiejr_12
(scaffolding protein)

Ruthiejr_13
(major capsid protein)

Ruthiejr_14
(head-to-tail adaptor)

Ruthiejr_15
(head-to-tail stopper)

Ruthiejr_16

Ruthiejr_17
(tail terminator)

Ruthiejr_18
(major tail protein)

Ruthiejr_19
(tail assembly chaperone)

Ruthiejr_20
(tail assembly chaperone)

Ruthiejr_21
(tape measure protein)

Ruthiejr_22
(minor tail protein)

Ruthiejr_23
(minor tail protein)

Ruthiejr_24

Ruthiejr_25
(minor tail protein)

Ruthiejr_26

Ruthiejr_27
(minor tail protein)

Ruthiejr_28

Ruthiejr_29
(minor tail protein)

Ruthiejr_30

Ruthiejr_31

Ruthiejr_32
(lysin A)

Ruthiejr_33
(lysin B)

Ruthiejr_34
(holin)

Ruthiejr_35

Ruthiejr_36

Ruthiejr_37
(MRE11 double-strand break endo/exonuclease)

Ruthiejr_38

Ruthiejr_40

Ruthiejr_41

Ruthiejr_42

Ruthiejr_43

Ruthiejr_44

Ruthiejr_45

Ruthiejr_46
(tyrosine integrase)

Ruthiejr_47

Ruthiejr_48
(immunity repressor)

Ruthiejr_49
(Cro protein)

Ruthiejr_50
(excise)

Ruthiejr_51

Ruthiejr_52

Ruthiejr_53

Ruthiejr_54

Ruthiejr_55

Ruthiejr_56

Ruthiejr_57

Ruthiejr_58

Ruthiejr_59

Ruthiejr_60
(HicA-like toxin)

Ruthiejr_61

Ruthiejr_62

Ruthiejr_63
(DnaQ-like DNA polymerase III subunit)

Ruthiejr_64

Ruthiejr_65
(Cas4 family exonuclease)

Ruthiejr_66

Ruthiejr_67

Ruthiejr_68

Ruthiejr_69

Ruthiejr_70

Ruthiejr_71
(NrdH-like glutaredoxin)

Ruthiejr_72
(DNA methyltransferase)

Ruthiejr_73

Ruthiejr_74
(HNH endonuclease)

Ruthiejr_75
(DNA primase/polymerase/helicase)

Ruthiejr_76

Ruthiejr_77
(HNH endonuclease)

Ruthiejr_78

Ruthiejr_79
(DNA binding protein)

Ruthiejr_80

Ruthiejr_81

Ruthiejr_82
(SprT-like protease)

Ruthiejr_83

Ruthiejr_84

Ruthiejr_85

Ruthiejr_86

Ruthiejr_87

Ruthiejr_88

Ruthiejr_89

Ruthiejr_90

Ruthiejr_91

Ruthiejr_92

Ruthiejr_93

Ruthiejr_94

Ruthiejr_95

Ruthiejr_96
(HNH endonuclease)

Ruthiejr_97
(O-methyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON526978
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2008HOPEruthiejrJRL
SEA Lysate Titer 6.4e9
Pitt Freezer Box# 3
Pitt Freezer Box Grid# 62
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload