Mycobacterium phage Ruthiejr
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Detailed Information for Phage Ruthiejr
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJennifer LaRoche
Year Found2008
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.7842 N, 86.09175 W Map
Discovery NotesFrom topsoil in a rural area, on the bank of a swampy pond, next to a walking bridge. 76° F

-Two different plaque morphologies were found (A and B)
-A had a diameter of 2mm
-B had a diameter of 7mm
-Plaque A (2008HOPEruthiejrJRL) was followed up
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 9, 2019
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage401
Genome length (bp)57858
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Capricorn (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Inky (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Spock (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionHope College
Annotation StatusBeing Annotated (Expected completion by 2/9/2020)
Plaque Notes-bulls eye (haloed)
-2-3mm in diameter
-incubation time: checked at 24 and 48 hours
-incubated at 37˚ C
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2008HOPEruthiejrJRL
SEA Lysate Titer 6.4e9
Pitt Freezer Box# 3
Pitt Freezer Box Grid# 62
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload