Mycobacterium phage Spock
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Detailed Information for Phage Spock
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKelly Coburn
Year Found2016
Location FoundLouisville, KY USA
Finding InstitutionSpalding University
ProgramKentucky Biomedical Infrastructure Network Small Genomes Discovery Program
From enriched soil sample?Yes
Isolation Temperature4°C
GPS Coordinates38.240278 N, 85.759167 W Map
Discovery NotesThe bacteriophage was obtained from a soil sample in the courtyard of the residence hall where I live on Spalding University's campus. The soil was taken approximately 6 centimeters below the top of the grass. It was 39 degrees Fahrenheit outside, and the ground was lightly topped with snow. My soil sample was then centrifuged in the laboratory. The suspended phage particles were filtered and plated on an agar plate with the bacterial medium host Mycobacterium smegmatis.
Naming NotesPhages are alien-like entities that invade the human body. I named my bacteriophage Spock because it is the name of my favorite alien on the TV show Star Trek.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 30, 2018
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage270
Genome length (bp)59709
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Capricorn (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Inky (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Spock (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionSpalding University
Annotation StatusIn GenBank
Plaque NotesThe bacteriophage plaques were lytic and approximately 1.5-2 millimeters in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Spock_1

Spock_2

Spock_3

Spock_4

Spock_6

Spock_7
(terminase, small subunit)

Spock_8
(terminase, large subunit)

Spock_9
(portal protein)

Spock_10
(capsid maturation protease)

Spock_11

Spock_12
(scaffolding protein)

Spock_13
(major capsid protein)

Spock_14
(head-to-tail adaptor)

Spock_15
(head-to-tail stopper)

Spock_16

Spock_17
(tail terminator)

Spock_18
(major tail protein)

Spock_19
(tail assembly chaperone)

Spock_20
(tail assembly chaperone)

Spock_21
(tape measure protein)

Spock_22
(minor tail protein)

Spock_23
(minor tail protein)

Spock_24
(minor tail protein)

Spock_25
(minor tail protein)

Spock_26
(minor tail protein)

Spock_27
(minor tail protein)

Spock_28
(minor tail protein)

Spock_29

Spock_30
(lysin A)

Spock_31
(Lysin B)

Spock_32
(holin)

Spock_33

Spock_34

Spock_35
(exonuclease)

Spock_36
(helix-turn-helix DNA binding domain)

Spock_37

Spock_38

Spock_39

Spock_40

Spock_41
(tyrosine integrase)

Spock_42
(membrane protein)

Spock_43
(immunity repressor)

Spock_44
(Cro (control of repressor`s operator))

Spock_45
(excise)

Spock_46

Spock_47
(membrane protein)

Spock_48

Spock_49
(helix-turn-helix DNA binding domain)

Spock_50
(WhiB family transcription factor)

Spock_51

Spock_52

Spock_53

Spock_54

Spock_55

Spock_56

Spock_57
(DnaE-like DNA polymerase III (alpha))

Spock_58

Spock_59

Spock_60

Spock_61
(exonuclease)

Spock_62

Spock_63

Spock_64

Spock_65

Spock_66
(NrdH-like glutaredoxin)

Spock_67
(membrane protein)

Spock_68
(HNH endonuclease)

Spock_69
(DNA primase/helicase)

Spock_70

Spock_71
(RusA-like resolvase (endonuclease))

Spock_72

Spock_73

Spock_74

Spock_75

Spock_76
(SprT-like protease)

Spock_77

Spock_78

Spock_79
(membrane protein)

Spock_80

Spock_81

Spock_82

Spock_83

Spock_84

Spock_85
(rtcB-like RNA ligase)

Spock_86

Spock_87

Spock_88

Spock_89

Spock_90

Spock_91
(antitoxin in toxin/antitoxin system, HicB-like)

Spock_92
(toxin in toxin/antitoxin system, HicA-like)

Spock_93

Spock_94

Spock_95

Spock_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN369742
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 28
Pitt Freezer Box Grid# B11
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload