Mycobacterium phage Nutello
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Detailed Information for Phage Nutello
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMatt Weiss
Year Found2012
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.78736 N, 86.103664 W Map
Discovery Notes1. 67 degrees Fahrenheit September morning.
2. moist, loamy and a little sandy soil sample 4.5 cm deep
3. It was exposed to sunlight.
4. Taken from urban environment near Hope College’s Grave Hall.
5. Found under decomposing mulch an inch from base of Russian Sage plant, Perovskia atriplicifolia.
Naming NotesNutella, the marvelous hazelnut spread, inspired the name Nutello on one fateful day in Spanish class before dinner.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 8, 2019
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2500
Genome length (bp)56439
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGGGGGCAT
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

Topper (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionHope College
Annotation StatusIn GenBank
Plaque NotesClear center just less than its diameter, with a slightly turbid ring on the outside.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Nutello_1

Nutello_2

Nutello_3

Nutello_4
(terminase)

Nutello_5
(portal protein)

Nutello_6
(MuF-like minor capsid protein)

Nutello_7

Nutello_8
(scaffolding protein)

Nutello_9
(major capsid protein)

Nutello_10
(head-to-tail adaptor)

Nutello_11
(head-to-tail stopper)

Nutello_12

Nutello_13
(tail terminator)

Nutello_14
(major tail protein)

Nutello_15
(tail assembly chaperone)

Nutello_16
(tail assembly chaperone)

Nutello_17
(tape measure protein)

Nutello_18
(minor tail protein)

Nutello_19
(minor tail protein)

Nutello_20
(minor tail protein)

Nutello_21
(minor tail protein)

Nutello_22
(minor tail protein)

Nutello_23
(minor tail protein)

Nutello_24
(minor tail protein)

Nutello_25

Nutello_26
(lysin A)

Nutello_27
(lysin B)

Nutello_28
(holin)

Nutello_29

Nutello_30

Nutello_31
(MRE11 double-strand break endo/exonuclease)

Nutello_32
(DNA binding protein)

Nutello_33

Nutello_34

Nutello_35
(Queuine tRNA-ribosyltransferase)

Nutello_36
(tyrosine integase)

Nutello_37

Nutello_38
(immunity repressor)

Nutello_39
(Cro (control of repressor's operator))

Nutello_40
(excise)

Nutello_41

Nutello_42

Nutello_43

Nutello_44

Nutello_45
(helix-turn-helix DNA binding domain, MerR-like)

Nutello_46
(WhiB family transcription factor)

Nutello_47

Nutello_48

Nutello_49
(DnaQ-like (DNA polymerase III subunit))

Nutello_50
(Cas4 family exonuclease)

Nutello_51

Nutello_52

Nutello_53

Nutello_54

Nutello_55
(NrdH-like glutaredoxin)

Nutello_56
(HNH endonuclease)

Nutello_57
(DNA primase/helicase)

Nutello_58
(RusA-like resolvase (endonuclease))

Nutello_59

Nutello_60
(DNA binding protein)

Nutello_61

Nutello_62

Nutello_63

Nutello_64

Nutello_65

Nutello_66
(polynucleotide kinase)

Nutello_67
(rtcB-like RNA ligase)

Nutello_68

Nutello_69

Nutello_70

Nutello_71

Nutello_72
(antitoxin in toxin/antitoxin system, HicB-like)

Nutello_73
(toxin in toxin/antitoxin system, HicA-like)

Nutello_74

Nutello_75
(DNA binding protein)

Nutello_76

Nutello_77
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOM913583
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.85*10^10 pfu/mL
Date of SEA Lysate Titering Dec 6, 2012
Pitt Freezer Box# 36
Pitt Freezer Box Grid# 51
Available Files
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload