Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Lysangelie I. Gonzalez-Santos |
Year Found | 2015 |
Location Found | Juana Diaz, PR USA |
Finding Institution | University of Puerto Rico at Cayey |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 17.995028 N, 66.516494 W Map |
Discovery Notes | Sample came from a crop field. The soil was dry. |
Naming Notes | I had made many attempts without success and was losing hope until I finally found LastHope. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 6, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 854 |
Genome length (bp) | 60934 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CTCGTAGGCAT |
GC Content | 66.9% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | K |
Subcluster | K1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Clear plaques, approximately 1.5 mm in diameter. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MF140416 |
Refseq Number | None yet |
Published in Paper | Anders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn |
Archiving Info |
Archiving status |
Not in Pitt Archives |
Available Files |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |