Mycobacterium phage LastHope
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Detailed Information for Phage LastHope
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLysangelie I. Gonzalez-Santos
Year Found2015
Location FoundJuana Diaz, PR USA
Finding InstitutionUniversity of Puerto Rico at Cayey
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates17.995028 N, 66.516494 W Map
Discovery NotesSample came from a crop field. The soil was dry.
Naming NotesI had made many attempts without success and was losing hope until I finally found LastHope.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage854
Genome length (bp)60934
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear plaques, approximately 1.5 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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LastHope_1

LastHope_2

LastHope_3

LastHope_4

LastHope_6

LastHope_7

LastHope_8
(terminase)

LastHope_9
(portal protein)

LastHope_10
(MuF-like minor capsid protein)

LastHope_11

LastHope_12
(scaffolding protein)

LastHope_13
(major capsid protein)

LastHope_14
(head-to-tail adaptor)

LastHope_15
(head-to-tail stopper)

LastHope_16

LastHope_17
(tail terminator)

LastHope_18

LastHope_20

LastHope_19

LastHope_21
(tape measure protein)

LastHope_22
(minor tail protein)

LastHope_23
(minor tail protein)

LastHope_24
(minor tail protein)

LastHope_25
(minor tail protein)

LastHope_26
(minor tail protein)

LastHope_27
(minor tail protein)

LastHope_28
(minor tail protein)

LastHope_29

LastHope_30
(lysin A)

LastHope_31
(lysin B)

LastHope_32
(holin)

LastHope_33

LastHope_34

LastHope_35
(MRE11 double-strand break endo/exonuclease)

LastHope_36

LastHope_37

LastHope_38

LastHope_39

LastHope_40

LastHope_41

LastHope_42

LastHope_43
(serine integrase)

LastHope_44

LastHope_45

LastHope_46
(immunity repressor)

LastHope_47

LastHope_48

LastHope_49

LastHope_50

LastHope_51

LastHope_52
(WhiB family transcription factor)

LastHope_53

LastHope_54

LastHope_55

LastHope_56

LastHope_57

LastHope_58

LastHope_59

LastHope_60
(DnaE-like DNA polymerase III)

LastHope_61

LastHope_62

LastHope_63

LastHope_64
(Cas4 family endonuclease)

LastHope_65

LastHope_66

LastHope_67

LastHope_68

LastHope_69

LastHope_70

LastHope_71

LastHope_72

LastHope_73
(DNA primase/helicase)

LastHope_74

LastHope_75
(RusA-like resolvase)

LastHope_76

LastHope_77

LastHope_78

LastHope_79

LastHope_80

LastHope_81

LastHope_82
(hydrolase)

LastHope_83
(SprT-like protease)

LastHope_84

LastHope_85

LastHope_86

LastHope_87

LastHope_88

LastHope_89

LastHope_90

LastHope_91
(RtcB-like RNA ligase)

LastHope_92

LastHope_93

LastHope_94

LastHope_95

LastHope_96

LastHope_97
(HicB-like antitoxin)

LastHope_98

LastHope_99

LastHope_100

LastHope_101

LastHope_102

LastHope_103
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140416
Refseq NumberNone yet
Published in PaperAnders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn
Archiving Info
Archiving status Not in Pitt Archives
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload