| Detailed Information for Phage TruffulaTree |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Meghan Perez |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.96611 N, 86.36306 W Map |
| Discovery Notes | This soil was collected from a compost pile at a depth of approximately 8 cm, the soil was moist and rich in decaying organic matter. The temperature outside was 80 degrees Fahrenheit. |
| Naming Notes | This phage is named TruffulaTree because of its resemblance to the trees in Dr. Seuss's book The Lorax. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 250 |
| Genome length (bp) | 60109 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | This phage produces round, turbid plaques that average 3 mm in diameter. Plaques are fully formed after 48 hours of incubation at 30 degrees celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253900 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUtruffulatreeMTP |
| SEA Lysate Titer |
5.3 x 10^10 pfu/mL |
| Date of SEA Lysate Titering |
Nov 18, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
D10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
