| Detailed Information for Phage TClif |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tyler Clifton |
| Year Found | 2022 |
| Location Found | bowling green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.000674 N, 86.428339 W Map |
| Discovery Notes | My environmental sample was taken right beside the kissing bridge on WKU campus, with coordinates 36°59'14.7258"N 86°27'7.833"W. My sample was taken on 8-29-22 at 5:13 pm. The temperature when my sample was taken was 27.22 degrees Celsius. The spot where it was taken was dry with the sample being taken from the topsoil around 3 inches deep. There was some debris with various leaves and sticks by the sample. There was also some roots from nearby trees mixed with the soil sample as well. |
| Naming Notes | This phage was named after its discoverer, Tyler Clifton. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 11, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1865 |
| Genome length (bp) | 61466 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGATGGCTT |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques are very small in size with some variation. The smallest plaques had a size of 2 mm in diameter with the larger plaques being around 5 mm in diameter. They also have a hazy shape with some plaques sticking to each other in clusters. My plaques also show signs of turbidity which means that it is lysogenic. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ201077 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.88E9 pfu / ml |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
H9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
