Mycobacterium phage Amgine
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Detailed Information for Phage Amgine
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCarmen Chamberlain
Year Found2016
Location FoundMI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature32°C
GPS Coordinates41.845976 N, 85.660045 W Map
Naming NotesThis phage was a bit of an enigma, hence Amgine, enigma spelled backwards.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 13, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1081
Genome length (bp)62236
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK6
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Amgine_1

Amgine_2

Amgine_3

Amgine_4

Amgine_5

Amgine_6
(terminase)

Amgine_7
(portal protein)

Amgine_8
(MuF-like minor capsid protein)

Amgine_9

Amgine_10
(scaffolding protein)

Amgine_11
(major capsid protein)

Amgine_12
(head-to-tail adaptor)

Amgine_13
(head-to-tail stopper)

Amgine_14

Amgine_15
(tail terminator)

Amgine_16
(major tail protein)

Amgine_17
(tail assembly chaperone)

Amgine_18
(tail assembly chaperone)

Amgine_19
(tape measure protein)

Amgine_20
(minor tail protein)

Amgine_21
(minor tail protein)

Amgine_22
(minor tail protein)

Amgine_23
(minor tail protein)

Amgine_24
(minor tail protein)

Amgine_25
(minor tail protein)

Amgine_26
(minor tail protein)

Amgine_27

Amgine_28
(lysin A)

Amgine_29
(lysin B)

Amgine_30
(holin)

Amgine_31

Amgine_32

Amgine_33
(MRE11 double-strand break endo/exonuclease)

Amgine_34

Amgine_35

Amgine_36

Amgine_37
(DNA methylase)

Amgine_38

Amgine_39

Amgine_40

Amgine_41
(tyrosine integrase)

Amgine_42

Amgine_43
(immunity repressor)

Amgine_44
(Cro protein)

Amgine_45
(excise)

Amgine_46

Amgine_47

Amgine_48

Amgine_49

Amgine_50

Amgine_51
(WhiB family transcription factor)

Amgine_52

Amgine_53

Amgine_54

Amgine_55

Amgine_56

Amgine_57
(DNA polymerase II exonuclease)

Amgine_58

Amgine_59
(Cas4 family exonuclease)

Amgine_60

Amgine_61

Amgine_62

Amgine_63

Amgine_64

Amgine_65
(NrdH-like glutaredoxin)

Amgine_66
(HNH endonuclease)

Amgine_67
(DNA primase/helicase)

Amgine_68

Amgine_69
(RusA-like resolvase)

Amgine_70

Amgine_71

Amgine_72

Amgine_73

Amgine_74

Amgine_75

Amgine_76

Amgine_77

Amgine_78

Amgine_79

Amgine_80

Amgine_81

Amgine_82

Amgine_83

Amgine_84

Amgine_85
(RtcB-like RNA ligase)

Amgine_86

Amgine_87

Amgine_88

Amgine_89

Amgine_90

Amgine_91

Amgine_92

Amgine_93

Amgine_94

Amgine_95

Amgine_96

Amgine_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF324915
Refseq NumberNone yet
Published in PaperAnders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn
Archiving Info
Archiving status Archived
Pitt Freezer Box# 34
Pitt Freezer Box Grid# B12
Available Files
GenBank File for PhameratorDownload