| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nana Yaa Amoh/SED |
| Year Found | 2015 |
| Location Found | Williamsburg, VA USA |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.226173 N, 76.738815 W Map |
| Discovery Notes | I found this in the woods behind the Sadler Center |
| Naming Notes | My last name is Amoh and it pronounced like the first syllable in the word ammunition |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 11, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1272 |
| Genome length (bp) | 61761 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 67.2% |
| Sequencing Notes | At position 23583 bp 21% of read have an A rather than consensus C. At position 25624 bp 41% of reads have a T rather than consensus C. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | phage morphology was clear |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140398 |
| Refseq Number | None yet |
| Published in Paper | Anders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.37X10^10 PFU/ml |
| Date of SEA Lysate Titering |
Nov 5, 2015 |
| Pitt Freezer Box# |
18 |
| Pitt Freezer Box Grid# |
H10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
