Mycobacterium phage Atiba
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| Detailed Information for Phage Atiba | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Shelby Hartley |
| Year Found | 2016 |
| Location Found | Hattiesburg, MS USA |
| Finding Institution | University of Southern Mississippi |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 31.32903 N, 89.333516 W Map |
| Discovery Notes | I found my phage underneath a patch of leaves which was shaded by trees. The soil was damp with lots of debris in it such as twigs and leaves. To collect my soil sample, I moved the leaves and collected the soil directly under the leaves. |
| Naming Notes | I named my phage Atiba after a horse that I used to ride at a summer camp I attended as a child. This horse was there every year and he was my favorite horse to ride. I like his color which reminds of cookies and cream. Also, I just like his name and think it is unique. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 599 |
| Genome length (bp) | 59556 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques were around 6mm in diameter, turbid and circular. Some had bullseyes in the center. All of my plaques were around the same size. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN234230 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 33 |
| Pitt Freezer Box Grid# | H1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
