| Detailed Information for Phage BananenDer11 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sally Dixon Molloy |
| Year Found | 2025 |
| Location Found | ORONO, ME United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 72°C |
| GPS Coordinates | 44.900879 N, 68.659144 W Map |
| Discovery Notes | The soil was collected from composted soil at the University of Maine Food Compost site. Daniel Dixon showed me how UMaine food waste is composted at this site to produce compost that is used in gardening on the UMaine campus. Direct isolations from these samples lead to lawns with too many plaques to count!!!! And more than five different plaque morphologies from a single soil extract. |
| Naming Notes | This phage was originally named BananasTBear after the UMaine mascot. But a pair of students also thought this was a cool name and decided to name their phage BananasTBear. I quickly pivoted and named my phage after the real bear mascot that my German grandfather used to help take care of at UMaine in the 1920s at UMaine. He told stories about Bananas the 11th racing around under the football stands eating candy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2026 |
| Sequencing Facility | Hubbard Center for Genome Studies at the University of New Hampshire |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Read Length | 250 bp |
| Approximate Shotgun Coverage | 55358 |
| Genome length (bp) | 61395 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 65.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | 3.0-mm plaques with small 1.0-mm clear centers surrounded by thick, very turbid rings.
Plaque density affects plaque morphology of BTB. At high plaque density plaques are smaller (1.0 mm in diameter) and have very thin turbid rings. At lower plaque density, plaque diameter increases to 3.0 mm and the turbid ring becomes much thicker. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
