| Detailed Information for Phage Beezoo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brian Zhu |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.93262 N, 86.40674 W Map |
| Discovery Notes | -From a compost pile
-24 degrees Celsius
-8 cm digging depth
-Sample collected on 09/20/2016 |
| Naming Notes | The phage name is the phonetics of the phage finder's first initial and last name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 27, 2017 |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 320 |
| Genome length (bp) | 60494 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | -Turbid plaques.
-3.2 mm
-Incubated for 48 hours at 30 degrees Celsius
|
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH371113 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUbeezooBZZ |
| SEA Lysate Titer |
2.7 x 10^11 pfu/mL |
| Date of SEA Lysate Titering |
Nov 3, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
B3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
