| Detailed Information for Phage Biglebops |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Braden Bigelow |
| Year Found | 2016 |
| Location Found | London, KY USA |
| Finding Institution | Somerset Community College |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.129 N, 84.0833 W Map |
| Discovery Notes | 12 inches below ground in garden
He was born in a garden after a really big snow break
Found in some mushy wet soil
|
| Naming Notes | I named him this because his name is amazing
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 23, 2016 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 270 |
| Genome length (bp) | 56454 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGGGGGCAT |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Somerset Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Clear Pretty circular
Very tiny had to have a lot to fill my plate
|
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH399770 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.4x10^11 pfu/mL |
| Date of SEA Lysate Titering |
Apr 28, 2016 |
| Pitt Freezer Box# |
28 |
| Pitt Freezer Box Grid# |
B1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
