| Detailed Information for Phage Boiiii |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kyle Brendan Cushman |
| Year Found | 2014 |
| Location Found | Midland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.788333 N, 86.105278 W Map |
| Discovery Notes | The soil was collected on 8/23/14, the air temperature was 28.3 degrees Celcius, the soil came from right on the surface, the soil was dry, and the it was collected in an urban environment (on Hope College's campus). |
| Naming Notes | The name Boiiii came from an inside joke with one of my classmates and I. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 4, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 37 |
| Genome length (bp) | 59907 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Benedictine University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque gave a bull's eye pattern (darker on the inside and lighter on the outside). After 24 hours of incubation the plaque diameter was 2 mm, and after both 48 and 72 hours of incubation the plaque diamater was 5 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK310505 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.10e10 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
E1 |
| Available Files |
| GenBank File for Phamerator | Download |