| Detailed Information for Phage BoostSeason |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | John Peroza |
| Year Found | 2017 |
| Location Found | Vero Beach, FL USA |
| Finding Institution | Indian River State College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 27.622778 N, 80.401611 W Map |
| Discovery Notes | Soil collected next to a canal. Sample was a mix of mostly darkish dirt, with some sand. Sample was collected from under a flowerbed, surrounded by insects of many kinds. |
| Naming Notes | One of my hobbies, which is racing cars. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 20, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1367 |
| Genome length (bp) | 58078 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGCGGCAT |
| GC Content | 68.2% |
| Sequencing Notes | sample contains mixed population, 16% Cluster L3 (not complete sequence)
Cluster K2 contains 12bp differences across genome, possibly 2 very similar phages |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Indian River State College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were circular, varying in size. Plaques were spread unequally throughout the plate, but with enough space between them for easy identification and quantification. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH834601 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
55 |
| Pitt Freezer Box Grid# |
A8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
