Mycobacterium phage CallaLilly
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Detailed Information for Phage CallaLilly
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlaina Arrollo
Year Found2024
Location FoundBowling Green, KY United State of America
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.986367 N, 86.454448 W Map
Discovery NotesPhage has a high titer and may capable of forming lysogens however those lysogens may be unstable.

Phage has a high concentration of DNA with my first elution having a concentration of 382.9 ng/ul while my second elution had an concentration of 106.2 ng/ul.

Phage appears to have a siphoviridae morphology type, and has potential for having "sticky heads" but this rarely appears within phage population. Phages tail is also quite flexible with many of them having a bend in the tail while viewing sample. Phage has an average length(capsid diameter + tail length) of 255.5354 nm ± 13.7009 nm.
Naming NotesWas named after the flower calla lily. It is one of my mother's favorite flowers and one of its meanings is resurrection and rebirth. With this meaning, I hope that phage and phage therapies developed from our research will help many find a rebirth and resurrection within their health and find themselves not departing from us as soon as before.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2025
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NextSeq 1000
Read TypeSingle-end reads
Read Length100 bp
Approximate Shotgun Coverage3024
Genome length (bp)59631
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesPhage may be capable of preforming lysogeny, however that may be an unstable lysogens.
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Bern (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

CallaLilly (K1)

Capricorn (K1)

CaseJules (K1)

Chavito (K1)

CheetoDust (K1)

Chris (K1)

Clipper (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MacKat (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

Teejan (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Doughnut (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

Lea83 (K3)

MacnCheese (K3)

Neighly (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Bobquesha (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Lebo14 (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

ViviJ (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Applecrisp (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

November (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionWestern Kentucky University
Annotation StatusQC Complete
Plaque NotesPlaques morphology is bullseye type plaques. Plaques are turbid and have an prominent halo and tend to be between about 3mm to 4mm in diameter and many do not exceed 4mm. Texture, size and color seem to be consistent throughout plates.
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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CallaLilly_1

CallaLilly_2

CallaLilly_3

CallaLilly_4

CallaLilly_5 — tRNA
(tRNA-Trp(cca))

CallaLilly_6

CallaLilly_7

CallaLilly_8
(terminase)

CallaLilly_9
(portal protein)

CallaLilly_10

CallaLilly_11

CallaLilly_12
(scaffolding protein)

CallaLilly_13
(major capsid protein)

CallaLilly_14
(head-to-tail adaptor)

CallaLilly_15
(head-to-tail stopper)

CallaLilly_16

CallaLilly_17
(tail terminator)

CallaLilly_18
(major tail protein)

CallaLilly_19
(tail assembly chaperone)

CallaLilly_20
(tail assembly chaperone)

CallaLilly_21
(tape measure protein)

CallaLilly_22
(minor tail protein)

CallaLilly_23
(minor tail protein)

CallaLilly_24

CallaLilly_25

CallaLilly_26

CallaLilly_27
(minor tail protein)

CallaLilly_28

CallaLilly_29

CallaLilly_30
(lysin A)

CallaLilly_31
(lysin B)

CallaLilly_32
(holin)

CallaLilly_33

CallaLilly_34
(helix-turn-helix DNA binding domain)

CallaLilly_35

CallaLilly_36

CallaLilly_37

CallaLilly_38

CallaLilly_39

CallaLilly_40
(secreted protein)

CallaLilly_41
(tyrosine integrase)

CallaLilly_42

CallaLilly_43
(immunity repressor)

CallaLilly_44
(helix-turn-helix DNA binding domain)

CallaLilly_45
(excise)

CallaLilly_46

CallaLilly_47

CallaLilly_48

CallaLilly_49
(helix-turn-helix DNA binding domain)

CallaLilly_50
(WhiB family transcription factor)

CallaLilly_51

CallaLilly_52
(secreted protein)

CallaLilly_53
(secreted protein)

CallaLilly_54

CallaLilly_55

CallaLilly_56

CallaLilly_57
(DnaE-like DNA polymerase III (alpha))

CallaLilly_58

CallaLilly_59

CallaLilly_60

CallaLilly_61
(Cas4 exonuclease)

CallaLilly_62

CallaLilly_63

CallaLilly_64

CallaLilly_65

CallaLilly_66
(NrdH-like glutaredoxin)

CallaLilly_67
(secreted protein)

CallaLilly_68
(HNH endonuclease)

CallaLilly_69
(DNA primase/helicase)

CallaLilly_70

CallaLilly_71
(RusA-like resolvase)

CallaLilly_72

CallaLilly_73

CallaLilly_74

CallaLilly_75

CallaLilly_76
(SprT-like protease)

CallaLilly_77

CallaLilly_78

CallaLilly_79
(DNA binding protein)

CallaLilly_80

CallaLilly_81
(helix-turn-helix DNA binding domain)

CallaLilly_82

CallaLilly_83

CallaLilly_84

CallaLilly_85
(rtcB-like RNA ligase)

CallaLilly_86
(terminase, large subunit (ATPase domain))

CallaLilly_87

CallaLilly_88

CallaLilly_89

CallaLilly_90

CallaLilly_91
(antitoxin in toxin/antitoxin system, HicB-like)

CallaLilly_92
(toxin in toxin/antitoxin system, HicA-like)

CallaLilly_93

CallaLilly_94

CallaLilly_95

CallaLilly_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 5.3 x 10^9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload