| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alaina Arrollo |
| Year Found | 2024 |
| Location Found | Bowling Green, KY United State of America |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.986367 N, 86.454448 W Map |
| Discovery Notes | Phage has a high titer and may capable of forming lysogens however those lysogens may be unstable.
Phage has a high concentration of DNA with my first elution having a concentration of 382.9 ng/ul while my second elution had an concentration of 106.2 ng/ul.
Phage appears to have a siphoviridae morphology type, and has potential for having "sticky heads" but this rarely appears within phage population. Phages tail is also quite flexible with many of them having a bend in the tail while viewing sample. Phage has an average length(capsid diameter + tail length) of 255.5354 nm ± 13.7009 nm. |
| Naming Notes | Was named after the flower calla lily. It is one of my mother's favorite flowers and one of its meanings is resurrection and rebirth. With this meaning, I hope that phage and phage therapies developed from our research will help many find a rebirth and resurrection within their health and find themselves not departing from us as soon as before. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3024 |
| Genome length (bp) | 59631 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Phage may be capable of preforming lysogeny, however that may be an unstable lysogens. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Plaques morphology is bullseye type plaques. Plaques are turbid and have an prominent halo and tend to be between about 3mm to 4mm in diameter and many do not exceed 4mm. Texture, size and color seem to be consistent throughout plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
5.3 x 10^9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
