| Detailed Information for Phage Capricorn |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | B'1B3P |
| Found By | Amanda Staples |
| Year Found | 2014 |
| Location Found | Upton, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.482447 N, 85.905523 W Map |
| Discovery Notes | Soil sample was collected beside the swing set in my front yard. A very rural area surrounded by cow farms. The soil was above a concrete encased 4x4 post that is continuously agitated by spades digging for worms. The color was light brown with little moisture (red clay predominates the area mostly). |
| Naming Notes | The Capricorn is the Zodiac sign of my oldest son. He's very sweet and sometimes seems so elusive. Just like him, only in the right light was this plaque visible. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 18, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 12697 |
| Genome length (bp) | 59708 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Small, very turbid plaques, clustered pretty close together. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK112537 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.1x10^9 |
| Date of SEA Lysate Titering |
Dec 3, 2014 |
| Pitt Freezer Box# |
52 |
| Pitt Freezer Box Grid# |
C8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
