| Detailed Information for Phage Dadosky |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Benjamin Hammond |
| Year Found | 2018 |
| Location Found | St. Pertersburg, FL USA |
| Finding Institution | Centre College |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 27.731796 N, 82.745992 W Map |
| Discovery Notes | Found near the hotel on 01/01/2018. Was an air temperature of 46 degrees Fahrenheit and cloudy with light showers. Soil collected from a depth of 9 cm. below ground level. Soil was partially moist and under 3 distinct layers of mulch. The granules were very fine almost like sand. The soil was harvested with a Gatorade powder scoop. It was next to a very highly trafficked area. The raised garden was surrounded on 1.5 sides by building and the other sides by cinderblock. It was about 1 meter away from the shrub, the only plant in the garden. |
| Naming Notes | The bacteriophage is named after my longtime girl friends last name. Named after Amara Dadosky |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 12, 2022 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 235 |
| Genome length (bp) | 62113 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 65.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Centre College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are about 2 mm in diameter, clear with a fuzzy boarder. They grow very quickly and all tests were conducted 20 hours after incubation. After 12 hours pfu would already be visible. The titer assay conducted on 01/15/2018 tuned up a pfu/mL amount that equaled 3.6x10^8. Run on a 10^-5 dilution plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP820461 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1x10^8 |
| Pitt Freezer Box# |
88 |
| Pitt Freezer Box Grid# |
C3 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
