| Detailed Information for Phage DismalFunk |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Holly Funkhouser |
| Year Found | 2015 |
| Location Found | South Mills, NC USA |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.50356 N, 76.353447 W Map |
| Naming Notes | My phage came from the Dismal Swamp and my last name is Funkhouser. Therefore I named my phage DismalFunk. It is a little part of my phage and a little part of me. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 15, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 891 |
| Genome length (bp) | 58129 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGCGGCAT |
| GC Content | 68.3% |
| Sequencing Notes | There are two variable positions in the sequencing data for this phage.
At position 20968, about 33% of the reads show an A instead of the published G.
At position 25813, about 50% of the reads show a C instead of the published T. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140408 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.18X10^10 pfu/mL |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
