Mycobacterium phage Dole
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Detailed Information for Phage Dole
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlex Dolehanty
Year Found2015
Location FoundLansing, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature32°C
GPS Coordinates42.756687 N, 84.611138 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 5, 2020
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage5255
Genome length (bp)60621
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionDrexel University
Annotation StatusIn GenBank
Plaque NotesSmall, round, turbid plaques.
MorphotypeSiphoviridae
Number of Genes98
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Dole_1

Dole_2

Dole_3

Dole_4

Dole_5 — tRNA
(tRNA-Trp(cca))

Dole_6

Dole_7

Dole_8
(terminase)

Dole_9
(portal protein)

Dole_10
(MuF-like minor capsid protein)

Dole_11

Dole_12
(scaffolding protein)

Dole_13
(major capsid protein)

Dole_14
(head-to-tail adaptor)

Dole_15
(head-to-tail stopper)

Dole_16

Dole_17
(tail terminator)

Dole_18
(major tail protein)

Dole_19
(tail assembly chaperone)

Dole_20
(tail assembly chaperone)

Dole_21
(tape measure protein)

Dole_22
(minor tail protein)

Dole_23
(minor tail protein)

Dole_24

Dole_25
(minor tail protein)

Dole_26

Dole_27
(minor tail protein)

Dole_28
(minor tail protein)

Dole_29

Dole_30
(lysin A)

Dole_31
(lysin B)

Dole_32
(holin)

Dole_33

Dole_34

Dole_35
(MRE11 double-strand break endo/exonuclease)

Dole_36

Dole_37

Dole_38

Dole_39

Dole_40

Dole_41

Dole_42

Dole_43

Dole_44
(tyrosine integrase)

Dole_45

Dole_46
(immunity repressor)

Dole_47
(helix-turn-helix DNA binding domain protein)

Dole_48
(excise)

Dole_49

Dole_50

Dole_51

Dole_52
(MerR-like helix-turn-helix DNA binding domain protein)

Dole_53
(WhiB family transcription factor)

Dole_54

Dole_55

Dole_56

Dole_57

Dole_58

Dole_59

Dole_60
(DnaE-like DNA polymerase III)

Dole_61

Dole_62

Dole_63

Dole_64

Dole_65

Dole_66
(Cas4 family exonuclease)

Dole_67

Dole_68

Dole_69

Dole_70

Dole_71

Dole_72
(NrdH-like glutaredoxin)

Dole_73
(HNH endonuclease)

Dole_74
(DNA primase/helicase)

Dole_75
(RusA-like resolvase)

Dole_76

Dole_77

Dole_78

Dole_79

Dole_80

Dole_81

Dole_82

Dole_83

Dole_84

Dole_85

Dole_86

Dole_87

Dole_88
(RtcB-like RNA ligase)

Dole_89

Dole_90

Dole_91

Dole_92

Dole_93

Dole_94
(HicB-like antitoxin)

Dole_95
(HicA-like toxin)

Dole_96

Dole_97

Dole_98

Dole_99
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ005674
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.95E10
Pitt Freezer Box# 19
Pitt Freezer Box Grid# D9
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload