| Detailed Information for Phage Feyre |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tuesday Melton, Grace Renois |
| Year Found | 2021 |
| Location Found | Arkadelphia, AR United States |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.193387 N, 93.078221 W Map |
| Discovery Notes | Our soil sample came from a compost pile in Arkadelphia, AR. After complementing 3 full plate titers, we concluded that we had a singular phage from our sample. |
| Naming Notes | We named our phage Feyre who is a very artistic and conflicted character. After exploring other phages, we found Rhysand who is very lytic. Rhysand and Feyre are married and it only made sense to name our beloved messy phage after her. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 12, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 628 |
| Genome length (bp) | 63558 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 64.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Our plaque showed a consistent size of about 0.4 cm through 3 full plate titers. For the most part, the plaques had a turbid look with blurred edges- only the occasional had a distinct outline. The centers were clear and became more turbid the farther from the center. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP059644 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7 * 10^9 pfu/mL |
| Pitt Freezer Box# |
135 |
| Pitt Freezer Box Grid# |
F4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
