Mycobacterium phage Gengar
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Detailed Information for Phage Gengar
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMichael Hetzel, Kelsy Mascorro
Year Found2013
Location FoundCorpus Christi, TX USA
Finding InstitutionDel Mar College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates27.793114 N, 97.402264 W Map
Discovery NotesDepth: 3 feet and 10 inches

Soil Type: Dense clay rich soil.

Temperature: 84 F

Naming NotesNamed after the Pokemon Gengar.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4124
Genome length (bp)61626
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content65.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Cluster Life CycleTemperate
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSmall Clustered Plaques. Fairly Fast growing. 2mm in Diameter.
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Gengar_1

Gengar_2

Gengar_3

Gengar_4 — tRNA
(tRNA-Trp(cca))

Gengar_5

Gengar_6
(terminase)

Gengar_7
(portal protein)

Gengar_8
(MuF-like minor capsid protein)

Gengar_9

Gengar_10
(scaffolding protein)

Gengar_11
(major capsid protein)

Gengar_12
(head-to-tail adaptor)

Gengar_13
(head-to-tail stopper)

Gengar_14

Gengar_15
(tail terminator)

Gengar_16
(major tail protein)

Gengar_17
(tail assembly chaperone)

Gengar_18
(tail assembly chaperone)

Gengar_19
(tape measure protein)

Gengar_20
(minor tail protein)

Gengar_21
(minor tail protein)

Gengar_22
(minor tail protein)

Gengar_23
(minor tail protein)

Gengar_24

Gengar_25
(minor tail protein)

Gengar_26

Gengar_27

Gengar_28

Gengar_29
(lysin A)

Gengar_30
(lysin B)

Gengar_31
(holin)

Gengar_32

Gengar_33

Gengar_34
(MRE11 double-strand break endo/exonuclease)

Gengar_35

Gengar_36

Gengar_37

Gengar_38
(serine integrase)

Gengar_39

Gengar_40

Gengar_41
(immunity repressor)

Gengar_42

Gengar_43

Gengar_44

Gengar_45

Gengar_46

Gengar_47
(DNA polymerase III sliding clamp)

Gengar_48

Gengar_49

Gengar_50

Gengar_51

Gengar_52
(DnaQ-like DNA polymerase III subunit)

Gengar_53

Gengar_54

Gengar_55
(Cas4 family exonuclease)

Gengar_56

Gengar_57

Gengar_58

Gengar_59

Gengar_60

Gengar_61
(HNH endonuclease)

Gengar_62
(HNH endonuclease)

Gengar_63
(DNA primase/polymerase)

Gengar_64
(DNA helicase)

Gengar_65
(holliday junction resolvase)

Gengar_66

Gengar_67

Gengar_68

Gengar_69

Gengar_70

Gengar_71

Gengar_72

Gengar_73

Gengar_74

Gengar_75

Gengar_76

Gengar_77

Gengar_78
(RNA ligase)

Gengar_79

Gengar_80

Gengar_81

Gengar_82

Gengar_83

Gengar_84

Gengar_85

Gengar_86

Gengar_87
(antitoxin)

Gengar_88

Gengar_89

Gengar_90

Gengar_91

Gengar_92

Gengar_93

Gengar_94

Gengar_95

Gengar_96

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX636165
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 1x10^9
Pitt Freezer Box# 1
Pitt Freezer Box Grid# H6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload