| Detailed Information for Phage Gengar |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Michael Hetzel, Kelsy Mascorro |
| Year Found | 2013 |
| Location Found | Corpus Christi, TX USA |
| Finding Institution | Del Mar College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 27.793114 N, 97.402264 W Map |
| Discovery Notes | Depth: 3 feet and 10 inches
Soil Type: Dense clay rich soil.
Temperature: 84 F
|
| Naming Notes | Named after the Pokemon Gengar. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4124 |
| Genome length (bp) | 61626 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 65.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Small Clustered Plaques. Fairly Fast growing. 2mm in Diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX636165 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1x10^9 |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
H6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
