| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gabriella Reyes |
| Year Found | 2015 |
| Location Found | Slidell, LA USA |
| Finding Institution | University of Southern Mississippi |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.291389 N, 89.723611 W Map |
| Discovery Notes | From the bank of Cross Gates neighborhood pond, also located behind an Autozone; compacted, moist soil, dug about 2 inches down to get sample; approximate air temperature: 90F |
| Naming Notes | Throughout these lab experiments, my partner and I were always hungry, and we thought that fresh agar plates smelled like ham, so we named our phage “Hammy” |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2914 |
| Genome length (bp) | 61812 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Our plaques were clear and measured about 3mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY087993 |
| Refseq Number | None yet |
| Published in Paper | Anders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.59 x 10^9 |
| Date of SEA Lysate Titering |
Nov 13, 2015 |
| Pitt Freezer Box# |
18 |
| Pitt Freezer Box Grid# |
B6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
