Mycobacterium phage Homura
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Detailed Information for Phage Homura
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDiana He
Year Found2012
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.645145 N, 90.314564 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 25, 2013
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage121
Sanger Finishing Reactions5
Genome length (bp)59708
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionNorthwest Indian College
Annotation StatusIn GenBank
Plaque Notesmedium, circular, clear plaques
MorphotypeSiphoviridae
Number of Genes94
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Homura_1

Homura_2

Homura_3

Homura_4

Homura_5 — tRNA
(codon recognized: UGG)

Homura_6

Homura_7

Homura_8
(terminase)

Homura_9
(portal protein)

Homura_10
(MuF-like minor capsid protein)

Homura_11

Homura_12
(scaffolding protein)

Homura_13
(major capsid protein)

Homura_14
(head-to-tail adaptor)

Homura_15
(head-to-tail stopper)

Homura_16

Homura_17
(tail terminator)

Homura_18

Homura_19

Homura_20

Homura_21
(tape measure protein)

Homura_22
(minor tail protein)

Homura_23
(minor tail protein)

Homura_24
(minor tail protein)

Homura_25
(minor tail protein)

Homura_26
(minor tail protein)

Homura_27
(minor tail protein)

Homura_28
(minor tail protein)

Homura_29

Homura_30
(lysin A)

Homura_31
(lysin B)

Homura_32
(holin)

Homura_33

Homura_34

Homura_35
(MRE11 double-strand break endo/exonuclease)

Homura_36

Homura_37

Homura_38

Homura_39

Homura_40

Homura_41
(tyrosine integrase)

Homura_42

Homura_43
(immunity repressor)

Homura_44
(helix-turn-helix DNA binding protein)

Homura_45
(excise)

Homura_46

Homura_47

Homura_48

Homura_49
(MerR-like helix-turn-helix DNA binding domain protein)

Homura_50
(WhiB family transcription factor)

Homura_51

Homura_52

Homura_53

Homura_54

Homura_55

Homura_56

Homura_57
(DnaE-like DNA polymerase III)

Homura_58

Homura_59

Homura_60

Homura_61
(Cas4 family endonuclease)

Homura_62

Homura_63

Homura_64

Homura_65

Homura_66
(NrdH-like glutaredoxin)

Homura_67

Homura_68
(HNH endonuclease)

Homura_69
(DNA primase/helicase)

Homura_70
(RusA-like resolvase)

Homura_71

Homura_72

Homura_73

Homura_74

Homura_75
(SprT-like protease)

Homura_76

Homura_77

Homura_78

Homura_79

Homura_80

Homura_81

Homura_82

Homura_83

Homura_84
(RtcB-like RNA ligase)

Homura_85

Homura_86

Homura_87

Homura_88

Homura_89

Homura_90
(HicB-like antitoxin)

Homura_91
(HicA-like toxin)

Homura_92

Homura_93

Homura_94

Homura_95
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH536821
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2012WUSLhomuraDH
SEA Lysate Titer 6x10^10 pfu/mL
Date of SEA Lysate Titering Oct 9, 2012
Pitt Freezer Box# 42
Pitt Freezer Box Grid# 15
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload