| Detailed Information for Phage Hyperbowlee |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sieun Ruth Lee |
| Year Found | 2014 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.7884 N, 86.1001 W Map |
| Discovery Notes | The phage was discovered on August 25, 2014 at 6:57pm. The ambient temperature was 82°F and the soil depth was 2cm. The soil was moist and located right underneath some bushes in front of Martha Miller Center on Hope College Campus. |
| Naming Notes | My friends have bestowed upon me the nickname hyperbowlee because I'm hyper most of the time and I had a bowl cut in 7th grade. It is also a pun on the word hyperbole. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 8, 2019 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1040 |
| Genome length (bp) | 59905 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | In GenBank |
| Plaque Notes | The pure phage stock of Hyperbowlee resulted in a clear, circular plaque with fuzzy edges (Figure 1). Its morphology is very similar to that of D29; however, their size differences assure us that it is distinct from D29, which is smaller than Hyperbowlee. The plaque is also a little more turbid than that of D29. Hyperbowlee’s plaque ranged from 2 to 2.5 mm in diameter at 24 hours and 4.2 to 5.2 mm in diameter at 48 hours. Its maximum detected size was 5.6 mm in diameter. Its growth rate was thus determined to be approximately 0.102 mm/hour. Hyperbowlee was isolated and purified at 32°C and was able to grow at 42°C. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OM818330 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.62E10 |
| Date of SEA Lysate Titering |
Oct 10, 2014 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
