| Detailed Information for Phage Joy99 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ho Yee Joyce Fung and Suchita Rastogi |
| Year Found | 2008 |
| Location Found | St Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.664567 N, 90.3179 W Map |
| Discovery Notes | Area that is used for a municipal mulch pile. Soil depth 1 cm at 70 degrees. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 12, 2012 |
| Approximate Shotgun Coverage | 157 |
| Genome length (bp) | 59837 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Tarleton State University |
| Annotation Status | In GenBank |
| Plaque Notes | Three-ring morphology; clear center spot, heavy thin middle ring, clouded, turbid outer ring/incubation 24 hours at 37 degree Celsius |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH536822 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2008WUSLjoy99SE |
| SEA Lysate Titer |
1.3e10 |
| Pitt Freezer Box# |
10 |
| Pitt Freezer Box Grid# |
4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
