Mycobacterium phage Juliette
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Detailed Information for Phage Juliette
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLaura Johnson
Year Found2012
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.78 N, 86.1 W Map
Naming NotesThis phage was named after Juliette from Romeo and Juliette as the literary Juliette tends to leave a wake of death behind her which is appropriate for a virus.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 8, 2019
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage37
Genome length (bp)58071
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content67.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of Mary
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes96
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Juliette_1

Juliette_2

Juliette_3

Juliette_4

Juliette_5

Juliette_6

Juliette_7

Juliette_8

Juliette_9
(terminase)

Juliette_10
(portal protein)

Juliette_11
(MuF-like minor capsid protein)

Juliette_12

Juliette_13
(scaffolding protein)

Juliette_14
(major capsid protein)

Juliette_15
(head-to-tail adaptor)

Juliette_16
(head-to-tail stopper)

Juliette_17

Juliette_18
(tail terminator)

Juliette_19
(major tail protein)

Juliette_20
(tail assembly chaperone)

Juliette_21
(tail assembly chaperone)

Juliette_22
(tape measure protein)

Juliette_23
(minor tail protein)

Juliette_24
(minor tail protein)

Juliette_25

Juliette_26

Juliette_27

Juliette_28
(minor tail protein)

Juliette_29

Juliette_30

Juliette_31

Juliette_32

Juliette_33
(lysin A)

Juliette_34
(lysin B)

Juliette_35
(holin)

Juliette_36

Juliette_37

Juliette_38
(MRE11 double-strand break endo/exonuclease)

Juliette_39

Juliette_40 — tRNA
(tRNA-Lys(ttt))

Juliette_41

Juliette_42

Juliette_43

Juliette_44

Juliette_45

Juliette_46

Juliette_47
(tyrosine integrase)

Juliette_48

Juliette_49
(immunity repressor)

Juliette_50
(Cro protein)

Juliette_51
(excise)

Juliette_52

Juliette_53

Juliette_54

Juliette_55

Juliette_56

Juliette_57

Juliette_58

Juliette_59

Juliette_60

Juliette_61
(HicA-like toxin)

Juliette_62

Juliette_63

Juliette_64
(DnaQ-like DNA polymerase III subunit)

Juliette_65

Juliette_66
(Cas4 family exonuclease)

Juliette_67

Juliette_68

Juliette_69

Juliette_70

Juliette_71

Juliette_72
(NrdH-like glutaredoxin)

Juliette_73
(DNA methylase)

Juliette_74

Juliette_75
(HNH endonuclease)

Juliette_76
(DNA primase/helicase)

Juliette_77

Juliette_78
(HNH endonuclease)

Juliette_79

Juliette_80

Juliette_81

Juliette_82

Juliette_83
(SprT-like protease)

Juliette_84

Juliette_85

Juliette_86

Juliette_87

Juliette_88

Juliette_89

Juliette_90

Juliette_91

Juliette_92

Juliette_93

Juliette_94

Juliette_95

Juliette_96
(HNH endonuclease)

Juliette_97
(methyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW601218
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2012HOPEjulietteLEJ
SEA Lysate Titer 1.75 * 10^11
Date of SEA Lysate Titering Oct 23, 2012
Pitt Freezer Box# 36
Pitt Freezer Box Grid# 48
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload