Mycobacterium phage Kraw
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Detailed Information for Phage Kraw
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMason Krawczynski and Kathleen Cornely
Year Found2023
Location FoundOak Bluffs, Martha's Vineyard, MA United States
Finding InstitutionProvidence College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates41.429167 N, 70.617222 W Map
Discovery NotesA soil sample was collected in Oaks Bluff, Massachusetts (Island of Martha's Vineyard), on Sunday, July 16, 2023 at 9:27 a.m. The soil was damp and approximately 4 centimeters into the ground in a garden bed. The weather was overcast and windy at 25 degrees C, with rain in the forecast later in the day.
Naming NotesThe phage name "Kraw" is a common nickname of mine, derived from the first four letters of my last name, "Krawczynski."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 22, 2024
Sequencing FacilityRhode Island INBRE Molecular Informatics Core
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypePaired-end reads
Read Length250 bp
Approximate Shotgun Coverage6076
Genome length (bp)57806
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content67.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Bern (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

CallaLilly (K1)

Capricorn (K1)

CaseJules (K1)

Chavito (K1)

CheetoDust (K1)

Chris (K1)

Clipper (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MacKat (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pisa4 (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

Teejan (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

Trice (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Doughnut (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

Lea83 (K3)

MacnCheese (K3)

Neighly (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Bobquesha (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Lebo14 (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

ViviJ (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Applecrisp (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

November (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionProvidence College
Annotation StatusQC Complete
MorphotypeSiphoviridae
Number of Genes94
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Kraw_1

Kraw_2

Kraw_3

Kraw_4

Kraw_5

Kraw_6

Kraw_7

Kraw_8

Kraw_9
(terminase)

Kraw_10
(portal protein)

Kraw_11
(capsid maturation protease)

Kraw_12

Kraw_13
(scaffolding protein)

Kraw_14
(major capsid protein)

Kraw_15
(head-to-tail adaptor)

Kraw_16
(head-to-tail stopper)

Kraw_17

Kraw_18
(tail terminator)

Kraw_19
(major tail protein)

Kraw_20
(tail assembly chaperone)

Kraw_21
(tail assembly chaperone)

Kraw_22
(tape measure protein)

Kraw_23
(Dit)

Kraw_24
(baseplate hub)

Kraw_25

Kraw_26
(minor tail protein)

Kraw_27

Kraw_28
(tail spike)

Kraw_29
(minor tail protein)

Kraw_30
(minor tail protein)

Kraw_31
(lysin A)

Kraw_32
(lysin B)

Kraw_33
(holin)

Kraw_34

Kraw_35
(helix-turn-helix DNA binding domain)

Kraw_36
(metallophosphatase)

Kraw_37

Kraw_38 — tRNA
(tRNA-Lys(ttt))

Kraw_39
(helix-turn-helix DNA binding domain)

Kraw_40

Kraw_41

Kraw_42

Kraw_43

Kraw_44

Kraw_45
(tyrosine integrase)

Kraw_46

Kraw_47
(immunity repressor)

Kraw_48
(Cro (control of repressor's operator))

Kraw_49
(excise)

Kraw_50

Kraw_51

Kraw_52

Kraw_53

Kraw_54

Kraw_55

Kraw_56

Kraw_57

Kraw_58

Kraw_59
(toxin in toxin/antitoxin system, HicA-like)

Kraw_60

Kraw_61

Kraw_62
(DnaQ-like (DNA polymerase III subunit))

Kraw_63

Kraw_64
(Cas4 family exonuclease)

Kraw_65

Kraw_66

Kraw_67

Kraw_68

Kraw_69

Kraw_70
(NrdH-like glutaredoxin)

Kraw_71
(DNA methyltransferase)

Kraw_72

Kraw_73
(HNH endonuclease)

Kraw_74
(DNA primase/polymerase/helicase)

Kraw_75

Kraw_76
(endonuclease VII)

Kraw_77

Kraw_78
(helix-turn-helix DNA binding domain)

Kraw_79

Kraw_80

Kraw_81
(SprT-like protease)

Kraw_82

Kraw_83

Kraw_84

Kraw_85

Kraw_86

Kraw_87

Kraw_88

Kraw_89

Kraw_90

Kraw_91

Kraw_92

Kraw_93

Kraw_94
(HNH endonuclease)

Kraw_95
(methyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 171
Pitt Freezer Box Grid# H4
Available Files
GenBank File for PhameratorDownload