| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tristan Tobias |
| Year Found | 2015 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.789249 N, 86.10076 W Map |
| Discovery Notes | Soil sample was collected by Alex Vroom from moist area near landscaping and cement steps of Dykstra Hall on Hope College campus. |
| Naming Notes | Krueger is named after the best biology and anatomy teacher, Mrs. Krueger. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 314 |
| Genome length (bp) | 60321 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGGGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are circular and symmetrical with approximately 2mm diameters. As concentration increased, plaque diameter decreased to about 1.5mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF324914 |
| Refseq Number | None yet |
| Published in Paper | Anders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.05 x 10^9 pfu |
| Date of SEA Lysate Titering |
Oct 22, 2015 |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
D11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
