Mycobacterium phage LastHope
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| Detailed Information for Phage LastHope | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lysangelie I. Gonzalez-Santos |
| Year Found | 2015 |
| Location Found | Juana Diaz, PR USA |
| Finding Institution | University of Puerto Rico at Cayey |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 17.995028 N, 66.516494 W Map |
| Discovery Notes | Sample came from a crop field. The soil was dry. |
| Naming Notes | I had made many attempts without success and was losing hope until I finally found LastHope. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 854 |
| Genome length (bp) | 60934 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques, approximately 1.5 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140416 |
| Refseq Number | None yet |
| Published in Paper | Anders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |