| Detailed Information for Phage Lavahound |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gavin White |
| Year Found | 2025 |
| Location Found | Harmony, ME United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 72°C |
| GPS Coordinates | 44.93807 N, 69.4738 W Map |
| Discovery Notes | Garden soil, 70°F, sunny, in a raised asparagus bed. Thank you Emily Ledue! |
| Naming Notes | My favorite card in Clash Royale. It helped me hit 10K trophies. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 144 |
| Genome length (bp) | 62367 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Two plaque morphologies were noted after numerous Titer Assays and it is determined that one phage is creating the two distinct plaque morphologies. One morphology includes a turbid plaque with an average diameter of 2.5mm. The second morphology includes a turbid pin-prick plaque with an average diameter of about <1mm |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |