| Detailed Information for Phage LeMond |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | DAJM, Sharkeisha |
| Found By | Jack Murphy and David Ahern |
| Year Found | 2014 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 47.665923 N, 117.403924 W Map |
| Discovery Notes | Soil taken at the base of a willow tree, next to a swampy lake. Located at the base of a hill near Hughes Hall on Gonzaga's campus. Soil appeared to be dry. Some small rocks and dry grass in sample. |
| Naming Notes | Named in honor of the great cyclist, Greg LeMond. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 107 |
| Genome length (bp) | 62515 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appeared cloudy and turbid. Plaques had a diameter of 2.0-2.5 millimeters. All plaques had a halo of approximately 0.3 millimeters. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH910038 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
16 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
