| Detailed Information for Phage Lea83 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Luciano D'Arcangelo and Christian Butler |
| Year Found | 2024 |
| Location Found | Orono, ME United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 44.89681 N, 68.66577 W Map |
| Naming Notes | Lea83 is short for "Lyse Em' All" based on the Metallica album "Kill Em' All". The 83 at the end of the name was the year that the Metallica album was released. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2025 |
| Sequencing Facility | Hubbard Center for Genome Studies at the University of New Hampshire |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Single-end reads |
| Read Length | 250 bp |
| Approximate Shotgun Coverage | 32350 |
| Genome length (bp) | 61105 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGATGGCAT |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | QC Complete |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
199 |
| Pitt Freezer Box Grid# |
C7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
