Mycobacterium phage Lebo14
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| Detailed Information for Phage Lebo14 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Molopo Johannes Lipali |
| Year Found | 2024 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.948962 S, 18.469738 E Map |
| Discovery Notes | The soil, collected from a depth of approximately 10 cm, came from a large outdoor flower pot containing various flowers. The soil was enriched with M. smegmatis for 24 hours to acquire plaques, and plaque plates were prepared by spotting and incubating them for 48 hours. |
| Naming Notes | The phage was named after my brother and late best friend, who shared the same name. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The initial plaques acquired from enriched soil filtrates, prior to the purification of the phages, were few and exhibited both clear and turbid morphology. After purification, there was a combination of turbid and clear plaques on the dilution plates prepared. A 10^-4 plate with both turbid and clear plaques was used to sample the phages for sequencing. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
