| Detailed Information for Phage Lebo14 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Molopo Johannes Lipali |
| Year Found | 2024 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.948962 S, 18.469738 E Map |
| Discovery Notes | The soil, collected from a depth of approximately 10 cm, came from a large outdoor flower pot containing various flowers.
The soil was enriched with M. smegmatis for 24 hours to acquire plaques, and plaque plates were prepared by spotting and incubating them for 48 hours. |
| Naming Notes | The phage was named after my brother and late best friend, who shared the same name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3478 |
| Genome length (bp) | 58230 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGCGGCCAT |
| GC Content | 68.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | TASK South Africa |
| Annotation Status | In GenBank |
| Plaque Notes | The initial plaques acquired from enriched soil filtrates, prior to the purification of the phages, were few and exhibited both clear and turbid morphology.
After purification, there was a combination of turbid and clear plaques on the dilution plates prepared. A 10^-4 plate with both turbid and clear plaques was used to sample the phages for sequencing. |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV755398 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
