| Detailed Information for Phage LilDirt |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brooke Jacomine & Jasmine Hamm |
| Year Found | 2025 |
| Location Found | Orono, ME United States of America |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 44.900879 N, 68.659144 W Map |
| Discovery Notes | Sunny, dry, sampled 6-7 inches below surface which was moist and wormy. |
| Naming Notes | We chose to name our phage "LilDirt" because it was found in a compost soil; we thought it was funny since it is akin to a rapper's name and our phage is small and to the point. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 9, 2026 |
| Sequencing Facility | Hubbard Center for Genome Studies at the University of New Hampshire |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Approximate Shotgun Coverage | 837 |
| Genome length (bp) | 61196 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 65.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Our plaques range from 2mm to 3mm in diameter. They present themselves as a large turbid ring surrounding a pin point center. |
| Has been Phamerated? | No |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
