| Detailed Information for Phage Llorens |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Daniela Lopez Llorens |
| Year Found | 2022 |
| Location Found | Oak Grove, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.64956 N, 87.43688 W Map |
| Discovery Notes | The soil sample was found near an electrical plant in Oak Grove, Kentucky. Soil was dry and sandy when collected with large pebbles. No decaying matter nearby. No prevalent nature nearby. No grass growing on top. |
| Naming Notes | The name of this phage is my mother's maiden name, which is also my second last name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 11, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1925 |
| Genome length (bp) | 59708 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | 3 mm in diameter. Little to no variety between plaques' shape. Slightly turbid pointing to lysogenic lifecycle. Opaque halos. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ559665 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.2 x 10^9 |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |