| Detailed Information for Phage Mdavu |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | NOMAHLUBI RADEBE |
| Year Found | 2018 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.818609 S, 30.944569 E Map |
| Discovery Notes | Discovered at westville campus in front of M block. After a day of incubation a plate full blown of plaques was observed. The phage multiplies in large numbers, thus requires a series of dilutions. |
| Naming Notes | Named the phage Mdavu, after my father, DAVID.
Its his nickname |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2310 |
| Genome length (bp) | 56443 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGGGGGCAT |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | cloudy irregular shaped plaques after 24hrs.
has titre at 10 |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN586025 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
