Mycobacterium phage MeaningOfLife
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Detailed Information for Phage MeaningOfLife
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByShannon Smith
Year Found2015
Location FoundBattle Creek, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature32°C
GPS Coordinates42.332089 N, 85.167904 W Map
Naming NotesI just wanted to be able to say I found the Meaningoflife.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 5, 2020
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage195
Genome length (bp)60432
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionVirginia State University
Annotation StatusIn GenBank
Plaque NotesIt creates about 5mm clear plaques
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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MeaningOfLife_1

MeaningOfLife_2

MeaningOfLife_3

MeaningOfLife_4

MeaningOfLife_5 — tRNA
(tRNA-Trp(cca))

MeaningOfLife_6

MeaningOfLife_7

MeaningOfLife_8
(terminase)

MeaningOfLife_9
(portal protein)

MeaningOfLife_10
(MuF-like minor capsid protein)

MeaningOfLife_11

MeaningOfLife_12
(scaffolding protein)

MeaningOfLife_13
(major capsid protein)

MeaningOfLife_14
(head-to-tail adaptor)

MeaningOfLife_15
(head-to-tail stopper)

MeaningOfLife_16

MeaningOfLife_17
(tail terminator)

MeaningOfLife_18
(major tail protein)

MeaningOfLife_19
(tail assembly chaperone)

MeaningOfLife_20
(tail assembly chaperone)

MeaningOfLife_21
(tape measure protein)

MeaningOfLife_22
(minor tail protein)

MeaningOfLife_23
(minor tail protein)

MeaningOfLife_24
(minor tail protein)

MeaningOfLife_25
(minor tail protein)

MeaningOfLife_26

MeaningOfLife_27
(minor tail protein)

MeaningOfLife_28
(minor tail protein)

MeaningOfLife_29

MeaningOfLife_30
(lysin A)

MeaningOfLife_31
(lysin B)

MeaningOfLife_32
(holin)

MeaningOfLife_33

MeaningOfLife_34

MeaningOfLife_35
(MRE11 double-strand break endo/exonuclease)

MeaningOfLife_36
(helix-turn-helix DNA binding protein)

MeaningOfLife_37

MeaningOfLife_38

MeaningOfLife_39

MeaningOfLife_40
(queuine tRNA-ribosyltransferase)

MeaningOfLife_41

MeaningOfLife_42
(tyrosine integrase)

MeaningOfLife_43

MeaningOfLife_44
(immunity repressor)

MeaningOfLife_45
(helix-turn-helix DNA binding domain protein)

MeaningOfLife_46
(excise)

MeaningOfLife_47

MeaningOfLife_48

MeaningOfLife_49

MeaningOfLife_50

MeaningOfLife_51
(WhiB family transcription factor)

MeaningOfLife_52

MeaningOfLife_53

MeaningOfLife_54

MeaningOfLife_55

MeaningOfLife_56

MeaningOfLife_57

MeaningOfLife_58
(DnaQ-like DNA polymerase III subunit)

MeaningOfLife_59

MeaningOfLife_60

MeaningOfLife_61

MeaningOfLife_62
(exonuclease)

MeaningOfLife_63

MeaningOfLife_64

MeaningOfLife_65

MeaningOfLife_66

MeaningOfLife_67
(NrdH-like glutaredoxin)

MeaningOfLife_68

MeaningOfLife_69
(HNH endonuclease)

MeaningOfLife_70
(DNA primase)

MeaningOfLife_71

MeaningOfLife_72
(RusA-like resolvase)

MeaningOfLife_73

MeaningOfLife_74

MeaningOfLife_75

MeaningOfLife_76

MeaningOfLife_77

MeaningOfLife_78

MeaningOfLife_79

MeaningOfLife_80

MeaningOfLife_81

MeaningOfLife_82

MeaningOfLife_83

MeaningOfLife_84

MeaningOfLife_85

MeaningOfLife_86

MeaningOfLife_87
(RtcB-like RNA ligase)

MeaningOfLife_88
(DNA binding protein)

MeaningOfLife_89

MeaningOfLife_90

MeaningOfLife_91

MeaningOfLife_92

MeaningOfLife_93
(HicB-like antitoxin)

MeaningOfLife_94
(HicA-like toxin)

MeaningOfLife_95

MeaningOfLife_96

MeaningOfLife_97

MeaningOfLife_98
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW862984
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.35*10^10
Pitt Freezer Box# 19
Pitt Freezer Box Grid# D12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload